三七皂甙单体2A-1-1对高血压患者血小板钙内流的影响  

Effects of 2A-1-1 on the Ca^(2+) Influx of Platelets From Essential Hypertension

作　　者：曾福仁 尹松梅[2] 周文胜 李泉清 关永源[3] 

机构地区：[1]湖南省老年医院,湖南长沙410001 [2]中山大学附属二医院 [3]中山大学中山医学院药理教研室

出　　处：《南华大学学报（医学版）》2005年第2期203-205,228,共4页Journal of Nanhua University(Medical Edition)

基　　金：广东省自然科学基金团队研究项目 (2 0 0 0 );广东省科技计划项目资助 (2 0 0 3C3 2 70 9);广东省重点科技攻关项目 (99M0 4810G)

摘　　要：目的　观察三七皂甙单体2A - 1 - 1对高血压患者血小板钙内流的影响,并探讨其对血小板受体操纵性钙通道(ROC)的作用。方法　采用Fura - 2 Am荧光探针双波长测定细胞胞浆游离钙离子浓度( [Ca2 + ]i)技术,以Nifedipine为对照观察2A - 1 - 1体外对环匹阿尼酸(CPA)介导的高血压患者血小板钙内流变化。结果　高血压患者血小板静息钙和钙内流均高于正常人(P <0 .0 5 ) ;2A - 1 - 1 ( 5、1 0、2 0 μmol L)可抑制CPA介导的高血压患者血小板钙内流(P <0 .0 5或0 .0 1 ) ;Nifedipine( 2 0 μmol L)不能抑制CPA介导的高血压患者或正常人血小板钙内流(P >0 .0 5 )。结论　2A - 1 - 1可能通过抑制高血压患者血小板ROC ,减少钙内流,从而降低其血小板活性。Objective: To observe the effect of Calcium channel's blocks on Ca 2+ influx of platelets from essential hypertension. And to explore its effect on the ROC(Receptor-dependent Ca 2+ channels) of platelets. Methods Fura-2 fluorescent technique was applied to detect cell [Ca 2+ ]i, and the effects of two types of Calcium channel's blocks on Ca 2+ influx of human platelets induced by CPA differently was observed. Results The resting [Ca 2+ ]i and Ca 2+ influx of platelet in essential hypertension group is higher than that in normal group. Nifedipine can not affect the Ca 2+ influx of platelets from essential hypertension or normal induced by CPA. 5,10 or 20 μmol/L 2A-1-1(purified component from Panax notoginsengs saponins)can refrain the Ca 2+ influx of platelets from essential hypertension induced by CPA. Conclusions 2A-1-1 can block the ROC(Receptor-dependent Ca 2+ channels) of the human platelets from essential hypertension,and can refrain Ca 2+ influx of platelets.

关 键 词：钙通道阻滞剂 高血压 血小板 钙内流 

分 类 号：R544.1[医药卫生—心血管疾病]