云锡矿尘致大鼠气管上皮细胞转化的研究  

STUDY ON TRAN8FORMATON OF RAT TRACHEAL EPITHELIALCELLS EXPOSED TO THE MINERAL DUST OF THE COMPANY OF TIN IN YUNNAN PROVINCE

作　　者：项芒 崔明珍 程书钧[1] 

机构地区：[1]北京市劳动卫生职业病防治研究所,中国医学科学院中国协和医科大学肿瘤研究所

出　　处：《中华劳动卫生职业病杂志》1994年第6期335-337,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：用大鼠气管上皮细胞体内—体外转化系统检测可能致癌的云南锡业公司（简称云锡）矿场沉积尘的致癌性。云锡矿尘、青石棉尘对Ｗｉｓｔａｒ大鼠气管灌注染尘，ｌ５ｄ后取其气管上皮细胞体外无血清培养，１周后用含血清的选择培养基培养，在接种大鼠气管上皮细胞５～６周后计数转化集落数。结果表明云锡矿尘剂全为３０～１２０ｍｇ／只、青石棉为２２，５ｍｇ／只，有剂量效应关系。提示云锡矿尘可能是云南锡业公司矿工肺癌高发的主要原因。并证明大鼠气管上皮细胞体内—体外转化系统是研究矿尘类物质致癌性的良好模型。n in vivo-vitro transformation system of rat tracheal eqithelial(RTE)cells was used to test the possible carcinogenic effectof the mineral dusts of Company of Tin in Yunnan(CTY).After intratracheal exposure to the mineral dusts or crocidolite for l5days,primary RTE cells were cultured in Ham’s Fl2 serurn free mediutm for 7 days then they were developed in a serum containedrnedium. Transformed colonies were counted after planting RTE cells for 5～6weeks. Trantformed colonies with dose rexponse.were induce by the mineral dust of CTY(30～120mg/rat)or crocidolite(22.5mg/rat)treatment.The study provided the evidenceof carcinogenic effect of the mineral dusts of CTY or crocidolite. The results suggest that the mincral dust might be a causative ef-fect of the development of human lung cancer in CTY.

关 键 词：矿尘 青石棉 大鼠 气管 上皮细胞 细胞转化 

分 类 号：R135.2[医药卫生—劳动卫生]