三元复合物光度法测定芦荟多糖  

The spectrophotometry of ternary complex to determine saccharides in aloe

作　　者：杜海燕[1] 庚梅[1] 孙家跃[1] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100037

出　　处：《化学试剂》2005年第6期347-348,367,共3页Chemical Reagents

基　　金：北京市自然科学基金 (2 0 3 2 0 0 7) ;北京市教委科技发展计划项目 (KM2 0 0 3 10 0 110 3 9)资助课题。

摘　　要：利用均匀试验考察了芦荟多糖与刚果红及人体免疫球蛋白生成三元复合物的最佳条件;研究了麦芽糊精、蔗糖和葡萄糖对显色反应的影响;建立了简便快速、常见糖类基本无干扰的测定芦荟多糖含量的新的分光光度法。对芦荟凝胶制品中的多糖进行分析,加标回收率在90 %～96% ,相对标准偏差≤5 %。The reaction conditions of the polysaccharides in aloe,CGR and CC were investigated by uniform design.The influence of maltodextrin,sucrose and glucose saccharides on chromogenic reaction was discussed.A new spectrophotometric method for determining polysaccharides in aloe was established,which was simple in operation,less time-consuming and suppressed nearly all the interferences from common saccharides.Determination of aloe-polysaccharides in aloe-products showed that the recovery ratio was 90%～96%,the relative standard deviation was ≤5%.

关 键 词：芦荟多糖 分光光度法 三元复合物 

分 类 号：Q539[生物学—生物化学]