PCR技术在农药学研究中的应用  

Application of polymerase chain reaction to pesticide science

作　　者：谭仕禄[1] 曾鑫年[1] 黎卓莹[1] 熊忠华[1] 张帅[1] 

机构地区：[1]华南农业大学农药与化学生物学教育部重点实验室,广东广州510642

出　　处：《植物保护》2005年第3期18-22,共5页Plant Protection

摘　　要：聚合酶链式反应具有灵敏度高、特异性强和操作简便等优点,是应用最为广泛的分子生物技术之一。目前,农药学越来越多的研究涉及分子和基因,PCR技术有着广泛的应用前景。本文简要介绍PCR技术的基本原理和特点,以及其在靶标生物抗药性研究、农药环境毒理、农药作用机理、生物农药的开发等方面的应用。Because of its ability to amplify particular DNA sequences in a relatively short time and its characters of high sensitivity, veracity and easy operation, the technology of polymerase chain reaction (PCR) has been widely applied in the biological fields and other related subjects since its birth, and now it has been further combined into more and more research fields. Pesticide science has been developing in the direction of microcosm, and more and more studies is focusing on the bio-macromolecules and genes. The keystones and components of PCR and its applications in the study of insect resistance, environmental toxicology of pesticide, mechanism of pesticide and the exploitation of bio-pesticides are introduced in this paper.

关 键 词：农药学 PCR技术 抗药性 环境毒理 作用机理 

分 类 号：S481[农业科学—农药学]