Cryopreservation of cynomolgus monkey (Macacafascicularis)spermatozoa in a chemically defined extender  

作　　者：Ya-HuiLi Ke-JunCai LeiSu MoGuan Xie-ChaoHe HongWang AndrasKovacs Wei-ZhiJi 

机构地区：[1]KunmingInstituteofZoology,ChineseAcademyofSciences,Kunming,Yunnan650223,China [3]GraduateSchooloftheChineseAcademyofSciences,Beijing100039,China／／CollegeofFoodScienceandTechnology,YunnanAgriculturalUniversity,Kunming,Yunnan650201,China [4]ResearchInstituteforAnimalBreedingandNutrition,2053Herceghalom,Hungary

出　　处：《Asian Journal of Andrology》2005年第2期139-144,共6页亚洲男性学杂志（英文版）

摘　　要：Aim:To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. Methods:Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris (mTTE) synthetic extender and glycerol as cryoprotectant.The effects of glycerol concentration (1%,3 %,5 %,10 % and 15 % [v/v]) and its equilibration time (10 min,30 min,60 min and 90 min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity.Results:The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P<0.05) for 5 % glycerol (42.95±2.55 and 50.39±2.42,respectively) than those of the other groups (1%:19.19±3.22 and 24.84±3.64;3 %:34.23±3.43 and 41.37±3.42;10 %: 15.68±2.36 and 21.39±3.14;15 %:7.47±1.44 and 12.90±2.18).The parameters for 30 min equilibration (42.95±2.55 and 50.39±2.42) were better (P<0.05) than those of the other groups (10 min:31.33±3.06 and 38. 98±3.31;60 min:32.49±3.86 and 40.01±4.18;90 min:31.16±3.66 and 38.30±3.78).Five percent glycerol and 30 min equilibration yielded the highest post-thaw sperm motility and head membrane integrity.Conclusion: Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender,which is related to the concentration and the equilibration time of glycerol.Aim:To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. Methods:Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris (mTTE) synthetic extender and glycerol as cryoprotectant.The effects of glycerol concentration (1%,3 %,5 %,10 % and 15 % [v/v]) and its equilibration time (10 min,30 min,60 min and 90 min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity.Results:The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P<0.05) for 5 % glycerol (42.95±2.55 and 50.39±2.42,respectively) than those of the other groups (1%:19.19±3.22 and 24.84±3.64;3 %:34.23±3.43 and 41.37±3.42;10 %: 15.68±2.36 and 21.39±3.14;15 %:7.47±1.44 and 12.90±2.18).The parameters for 30 min equilibration (42.95±2.55 and 50.39±2.42) were better (P<0.05) than those of the other groups (10 min:31.33±3.06 and 38. 98±3.31;60 min:32.49±3.86 and 40.01±4.18;90 min:31.16±3.66 and 38.30±3.78).Five percent glycerol and 30 min equilibration yielded the highest post-thaw sperm motility and head membrane integrity.Conclusion: Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender,which is related to the concentration and the equilibration time of glycerol.

关 键 词：CRYOPRESERVATION MACACAFASCICULARIS chemically defined extender GLYCEROL 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]