Graves病患者T细胞受体β链基因多态性研究  

POLYMORPHISM OFT CELL RECEPTOR β-CHAIN GENE AND SUSCEPTIBILITY TO GRAVES′DISEASE

作　　者：马骏先[1] 孙荣[1] 罗素珍 王琴琴[1] 郁忠勤[1] 申功庆 许曼音[1] 罗敏[1] 张达青[1] 陈家伦[1] 

机构地区：[1]上海第二医科大学上海市内分泌研究所

出　　处：《中华内分泌代谢杂志》1994年第1期5-6,共2页Chinese Journal of Endocrinology and Metabolism

摘　　要：本文用Ｔｒｉｔｏ－溶菌酶法制备质粒ＤＮＡ，氯化铯梯度离心，经ＨｉｎｄⅢ和ＥｃｏＲＩ双酶切后，电泳分离６８０ｂｐＤＮＡ片段，得到Ｔ细胞受体β链（ＴＣＲβ）Ｃ区探针。抽提２８例Ｇｒａｖｅｓ病（ＧＤ）患者和４２例正常人外周血ＤＮＡ，经ＢｇｌⅡ酶切后，Ｓｏｕｔｈｅｒｎ转移至尼龙膜上，与按随机法标记的探针杂交。结果发现有三种基因表现型即１０／１０Ｋｂ，１０／９．２Ｋｂ，９．２／９．２Ｋｂ，其中ＧＤ患者１０／９．２Ｋｂ杂合子出现频率明显高于正常对照组（分别为０．７１，０．３８，Ｐ＜０．０１），而ＧＤ患者１０／１０Ｋｂ纯合子出现频率则明显低于正常对照组（分别为０．０７，０．３１，Ｐ＜０．０１）。Polymorphism in the genes encoding the T cell receptor βchain (TLRβ)has been detected by molecular genotyping analysis inpatients with Graves′disease(GD)and in normalcontrol.The enzyme Bgl II revealed restriction-fragment-length polymorphism inthese samples.Asignificant increase in the frequency of 10.0- and 9.2-Kliobase heterozygous phenotype was found in GD(71.43%).as compared with 38.10%in the normal subjects (P<0.01).These results suggest that TCRβchain genes are associ-ated with susceptibility to GD and may be most pronounced in those individuals who ate HLA-DR,positive.

关 键 词：β链基因 多态性 甲亢 T细胞 

分 类 号：R581.1[医药卫生—内分泌]