血清生长激素结合蛋白测定方法及其临床意义  

HUMAN SERUM GHBP ASSAY AND ITS CLINICAL SIGNIFICANCE

作　　者：张宇瑾[1] 王德芬[1] 史爱兰[1] 曾畿生[1] 

机构地区：[1]上海第二医科大学附属瑞金医院儿内科,上海铁道医学院附属甘泉医院遗传室

出　　处：《中华内分泌代谢杂志》1994年第3期170-173,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：作者研究了炭吸收法分离技术测定血清生长激素结合蛋白（ＧＨＢＰ）的相关因素，提出了理想的本实验室的检测条件。本法灵敏度为０．９％／１０μｌ血清，批内ＣＶ为２．５％，批间ＣＶ为６．０％。并用此法对正常青春发育期儿童１０６例，ＧＨＤ２０例，ＧＨＮＤ２４例，肥胖症１０例，Ｌａｒｏｎ型侏儒症１例，进行了血清ＧＨＢＰ测定。其中４例矮小儿童同时测夜睡眠５ｈ血清ＧＨ分泌相及ＧＨＢＰ水平的动态观察。In this report the authors studied the relevant factors in the dextran-coated charcoal technique for separating the bound-hGHfrom the free-hGH,and established an optimized condition, The sensitivity was 0.9%/10 μ1 serum. Intra-and inter-assay coeffi-cients of variation were 2.5%and 6.0%respectively. UsinR this method the authors measured the serum GHBP in 106 normal pu-bertal children , among them 20 with GHD, 24 with GHND,10 with obesity and one with Laron’s syndrome. In 4 children withshort-statue 5-hour night time GH and GHBP profiles were measured.

关 键 词：生长激素 结合蛋白 血清诊断 

分 类 号：R446.112[医药卫生—诊断学]