静脉免疫球蛋白对新生儿脐带血淋巴细胞免疫抑制机制的研究  

Mechanism of inhibitory effect of intravenous immunoglobulin on neonatal umbilical cord blood lymphocytes

作　　者：郝轶群[1] 陈同辛[1] 朱亚忠[1] 李庆生[1] 

机构地区：[1]上海第二医科大学附属新华医院上海市儿科医学研究所免疫/肿瘤研究室,200092

出　　处：《中华儿科杂志》2005年第6期438-443,共6页Chinese Journal of Pediatrics

基　　金：国家自然科学基金资助项目(39600158)

摘　　要：目的从脐带血单个核细胞(CBMC)和CD3+T淋巴细胞膜表面CD25、CD45RA、CD45RO分子表达的角度,探讨静脉免疫球蛋白(IVIG)对新生儿免疫功能的抑制机制。方法利用IVIG和植物血凝素(PHA)不同组合对CBMC或CD3+T淋巴细胞进行刺激培养,再利用四色免疫荧光抗体标记-流式细胞技术检测细胞表面CD25、CD45RA、CD45RO分子的表达情况。结果IVIG可以抑制PHA诱导的CBMC的活化,表现为CD25分子表达的明显抑制;并且随着CD25分子表达的抑制,CD4+细胞表面的CD45RO分子的表达也被抑制,阻止了CBMC中的CD4+CD45RA+细胞向CD4+CD45RO+细胞转换。IVIG也可以抑制PHA诱导的脐带血CD3+T淋巴细胞CD25分子和CD45RO分子的表达,但这种抑制程度远远不如对CBMC作用明显。结论IVIG可以抑制脐带血T淋巴细胞的活化过程,这种抑制作用除了与IVIG对T淋巴细胞的直接作用外,还可能通过了其他免疫细胞或免疫分子的间接介导。IVIG对CD4+CD45RO+T淋巴细胞的抑制作用可能是IVIG抑制B淋巴细胞免疫球蛋白释放的重要机制之一。新生儿期应用IVIG有可能使免疫功能低下加重。Objective The expression of CD25, CD45RA, CD45RO on umbilical cord blood mononuclear cells (CBMCs) and CD3 + T lymphocytes was investigated to explore the mechanism of immunosuppressive effects of intravenous immunoglobulin on neonatal immune function. Methods Umbilical cord blood mononuclear cells and CD3 + T lymphocytes isolated from 8 neonates were studied. The expression of CD25, CD45RA, CD45RO on umbilical cord blood mononuclear cells (CBMCs) and CD3 + T lymphocytes induced with various stimuli of different combinations of IVIG and phytohemagglutinin (PHA) including ① control group, ② PHA activation group, ③ IVIG pre-inhibition group, ④ PHA pre-activation group, ⑤ PHA+IVIG group was measured with four-color immunofluorescence antibodies staining-flow cytometric technique. The results were also compared with peripheral blood mononuclear cells of 8 adults (PBMCs). Results IVIG inhibited the PHA-induced proliferation of CBMCs as reflected by the decreased expression of CD25 and CD45RO. The amounts of CD25 + and CD4 +CD45RO + CBMCs reached 77.52%±2.31% and 64.29%±3.09% after PHA use. But a decreased response in CD25 + (7.66%±1.20% and 7.78%±1.46%) and CD4 +CD45RO + CBMC (3.18%±1.90% and 3.11%±0.08%) was observed when IVIG was added in both IVIG pre-inhibition group and PHA+IVIG group. As compared with PBMCs, IVIG failed to induce the increase of the expression of CD45RA in CBMCs whereas CD45RA + PBMCs increased from 54.93%±3.63% to 72.77%±0.39% in IVIG pre-inhibition group. Moreover, IVIG inhibited the expression of CD25 and CD45RO on cord blood CD3 + T lymphocytes no matter whether they were activated with PHA or not. The amounts of CD25 + and CD4 +CD45RO + CD3 + T lymphocytes reached 97.92%±2.19% and 80.41%±5.57% after PHA use. But a decreased response in CD25 + CBMCs (77.29%±0.63%, 51.48%±1.85% and 62.73%±1.24%) and CD4 +CD45RO + CD3 + T lymphocytes (35.47%±2.55%, 40.14%±1.16% and 36.41%±2.96%) was observed when IVIG was added in IVIG pre-inhibition group, PHA pre-activation group an

关 键 词：静脉免疫球蛋白 免疫抑制机制 血淋巴细胞 新生儿脐带 CD45RO分子 CD25分子表达 CD45RA^+细胞 T淋巴细胞 脐带血单个核细胞 CD3^+ IVIG 细胞免疫球蛋白 CD4^+ CBMC 荧光抗体标记 免疫功能低下 细胞表面 抑制作用 植物血凝素 

分 类 号：R722.1[医药卫生—儿科]