HPLC-ELSD法测定浙贝母中主要生物碱的含量  

Determination of peimine and peiminine in Fritillaria thunbergii by HPLC-ELSD

作　　者：薛燕[1] 顾好粮 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050 [2]沃特斯中国有限公司-北京代表处,北京100005

出　　处：《药学学报》2005年第6期550-552,共3页Acta Pharmaceutica Sinica

基　　金：科技部中央级科研院所科技基础性工作专项研究课题.

摘　　要：目的　利用HPLC- ELSD测定贝母类药材中的贝母素甲和贝母素乙。方法　XTerraRP18色谱柱( 150mm×3 9mmID, 5μm),流动相为乙腈10mmol·L-1 NH4HCO3 (浓氨水调至pH 10 .10) =A∶B系统,梯度洗脱,以ELSD检测。结果　贝母素甲在1 09-4 36μg,贝母素乙在1 .04-4 .16μg与峰面积的对数值呈线性关系,回收率分别为98. 96% (n=4),RSD1 01%; 98. 40% (n=4),RSD2 .63%。结论　本方法能够很好地测定浙贝母药材中的主要生物碱贝母素甲、贝母素乙,可用于浙贝母药材的质量控制。Aim To determine peimine and peiminine in Fritillaria drug simultaneously by RP-HPLC-ELSD. Methods HPLC was carried out with a Waters Alliance, Model 2690,equipped with XTerra RP18 column (150 mm×3.9 mm ID, 5 μm) and evaporated light scattering detector. The mobile phase (acetonitrile-10 mmol·L^-1 NH ~4HCO ~3 adjusted to pH 10.10 by ammonia solution) was eluted in gradient mode. Results The recoveries of peimine and peiminine were 98.96% (n=4), with RSD 1.01% and 98.40% (n=4), with RSD 2.63%, respectively. Conclusion The method is simple, sensitive and reliable. It can be used for quantitative determination of Fritillaria drug.

关 键 词：浙贝母 贝母素甲 贝母素乙 HPLC-ELSD 

分 类 号：R917[医药卫生—药物分析学]