输注TGF-β1基因修饰的供体脾细胞诱导同种大鼠移植心脏耐受的实验研究  

Transforming growth factor β1gene-modified donor spleen cell transfusion induced allo-heart tolerance in rats

作　　者：成俊[1] 夏穗生[1] 史晓峰[1] 谢森[2] 唐礼功[2] 

机构地区：[1]华中科技大学同济医学院器官移植研究所,武汉市430030 [2]广州军区武汉总医院泌尿外科,430070

出　　处：《实用医学杂志》2005年第12期1253-1256,共4页The Journal of Practical Medicine

摘　　要：目的:研究TGF-β1修饰的供者脾细胞特异性输注对同种大鼠移植心脏耐受的诱导及效果。方法:建立同种大鼠异位心脏移植模型,用脂质体转染TGF-β1基因修饰供者脾细胞;观察TGF-β1修饰的脾细胞输注组,单纯脾细胞输注组和假处理组移植心脏存活天数和病理改变。结果:建立了稳定转染TGF-β1基因的脾细胞;TGF-β1修饰的脾细胞特异性输注可明显延长移植心脏存活时间(17.0±3.3)d,与单纯脾细胞输注组(7.0±1.1)d和假处理组(6.3±1.0)d比较,差异有显著性(P<0.05);而且可明显减轻同种移植心脏的病理损伤。结论:输注TGF-β1基因修饰的供体脾细胞可诱导同种大鼠移植心脏的耐受产生。Objective T o investigate whether TGF-β1gene-modified donor spleen cell tr ansfusion could induce tolerogenicity and prolong allogra ft survival in rat heterotopic heart transplantation.Methods Rat heterotopic heart allograft model was established ,donor spleen cells were genetically modified wit h TGF-β1gene by the liposo me method,surviv al duration and histological changes o f TGF-β1gene-modifi ed donor spleen cells g roup,donor spleen cells group and sh am group were meas ured.Results Stable TGF-β1-transduced spleen cells were esta blished.TGF- β1gene-modified donor spleen cell transfusion had a modestly but signi ficant ly survival prolongation wit h a mean allograft survival of(17.0±3.3)d ays,compared with(7.0±1.1)days in unmodifeied spleen cells gro up and (6.3±1.0)days in sham group(P<0.05).Furthermore,the histologic altera tions were markedly abated by TGF-β1gene-modified donor spleen cell tr ansfusion.Conclusion TGF-β1-modified donor spleen cell transfusion could induce rat heart allogra ft immune tolerance.[

关 键 词：移植心脏 基因修饰 脾细胞诱导 同种 大鼠 实验研究 耐受 TGF-β1基因 脾细胞输注 特异性输注 供者脾细胞 心脏移植模型 脂质体转染 供体脾细胞 病理改变 稳定转染 存活时间 病理损伤 显著性 单纯 

分 类 号：R654.2[医药卫生—外科学] R735.7[医药卫生—临床医学]