M-Dot-ELISA检测麻风家内接触者血清中麻风杆菌特异性抗原  

Detection of M. leprae-specific Antigen with M-Dot-ELISA in Sera from Household Contacts of Leprosy Patients

作　　者：曹元华[1] 和泉真藏 吴勤学[1] 叶干运[1] 川津邦雄 M.D.Amiruddin MauroF.Mendes 侯伟[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京邮政编码210042 [2]日本厚生省国立多摩研究所 [3]印度尼西亚Hasanuddin大学 [4]巴西SocieddeFilantropicalHumanitas

出　　处：《中华皮肤科杂志》1994年第2期67-69,共3页Chinese Journal of Dermatology

摘　　要：用改良酶联免疫斑点１５试验（简称 Ｍ－Ｄｏｔ－ＥＬＩＳＡ）对麻风家内接触者（ＨＣ）血清进行了麻风杆菌特异性酚酚糖脂Ｉ（ＰＧＬ－Ｉ）抗原检测。７５例ＨＣ均经麻风杆菌明胶微粒凝集试验（ＭＬＰＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）证实为麻风杆菌特异性抗体阳性者。结果表明：①７５例ＨＣ中抗原阳性者１６例，阳性率为２１％。接触多菌型麻风（ＭＢ）者的抗原阳性率（２８％）明显高于接触少菌型麻风（ＰＢ）者（０％），两者之间有极显著性差异（ Ｐ ＜０．０１ ）。②抗体弱、强阳性组的抗原阳性率和抗原量之间亦有极显著差异（Ｐ＜０．０１）。③４０例正常人及１０例经ＭＬＰＡ和ＥＬＩＳＡ检测证实特异性抗体阳性的非麻风患者血清标本，抗原检测均阴性。We report the results of detecting M. lepraespecific antigen--phenolic glycolipid I (PGL--I) with modified M-Dot-ELISA in sera from 75 cases of household contacts (HC)of leprosy patients, which were all seropositive by Gelatin Particle Agglutination Test (MLPA)and ELISA. The results indicate that: (1 ) 16/75 (21. 3 % ) are antigen positive. The rates of positivity in HC of MB patients are much higher than those in HC of PB patients,the difference (P < 0. 01 ) between them is significant. (2 ) Positive rates and levels of PGL-I in HCs are associated with levels of antibody against PGL--I in sera from HCs. There are significant differences in antigen positive rates and levels between weak and strong antibody positive groups of HC (P <0. 01). (3)The sera from 40 normal controls and 10 nonleprosy patients with positive anti--PGL--I antibody by MLPA and ELISA are antigen negative.

关 键 词：麻风 抗原 麻风杆菌 ELISA 

分 类 号：R755.03[医药卫生—皮肤病学与性病学]