2,5-己二酮诱导的PC12细胞损伤和凋亡  

Injury and apoptosis of PC12 cells induced by 2,5-hexanedione

作　　者：孙凌聪[1] 杨国成[1] 夏腊菊[1] 朱汉明[1] 张澄宇[1] 刘少平[1] 

机构地区：[1]武汉大学医学院生物化学与分子生物学系,湖北武汉430071

出　　处：《中国职业医学》2005年第3期17-19,共3页China Occupational Medicine

摘　　要：目的建立2,5己二酮诱导的PC12细胞凋亡模型。方法以PC12细胞为神经元的细胞模型,将2,5己二酮(30、40、50mmol/L)加入培养的PC12细胞中,四甲基偶氮唑盐检测细胞活性,Hochest33258观察细胞核变化,琼脂糖凝胶电泳检测DNA断裂、流式细胞仪检测细胞凋亡率,比较各组间的差异。结果随2,5己二酮浓度增加,细胞存活率下降(P<0.01);荧光显微镜观察有特征性的凋亡细胞核;琼脂糖凝胶电泳有明显的DNAladder;流式细胞仪检测凋亡率随2,5己二酮浓度的增大而增加(P<0.05)。结论一定浓度的2,5己二酮有导致PC12细胞损伤和凋亡的作用。Objective To build the model of injury and apoptosis of PC12 cells induced by 2,5-hexanedone (2,5-HD). Methods With PC12 cells as the model of neurons, different concentrations of 2,5-hexanedione (30、40、50 mmol/L) were added into the culture of PC12 cells, and then cell viability was tested with MTT; change of nucleolus stained with Hoches 33258 was observed with fluoroscope; DNA fragment was observed with agarose gel electrophoresis; apoptosis ratio was tested with flow cytometry (FACS); and the differences between each groups were compared. Results Cell viabilities decreased with the concentrations of 2, 5-HD increasing (P<0.01). Hochest33258 staining could distinguish apoptotic cells as well as normal cells. There were obvious DNA fragments under agarose gel electrophoresis. The apoptosis ratio increased with the concentrations of 2,5-hexanedione getting higher (P<0.05). Conclusion 2,5-hexanedione could induce injury and apoptosis to the PC12 cells.

关 键 词：2 5-己二酮 PC12细胞 凋亡 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]