离体胎大脑皮质细胞存活时间的观察  

The observation of surviving-time of isolated human fetal cortical cells

作　　者：张峰[1] 祁岩超[1] 卢发根 卜夏[1] 张鹏远[1] 王燕房 苏芳忠[1] 

机构地区：[1]河南医科大学第二附属医院神经外科,河南省肿瘤研究所细胞生物窒,河南省邓州市人民医院

出　　处：《中华器官移植杂志》1994年第3期98-99,共2页Chinese Journal of Organ Transplantation

摘　　要：取１２例胎龄为１６～２６周的死胎大脑皮质，用机械分离法制成细胞悬液，二乙酸荧光素摄取试验和台盼蓝排斥试验检测细胞活度。结果显示，胎儿离体１小时时，胎龄为１６～２０周的胎脑皮质细胞活度为４８．３８％，２１～２４周的为３８．７１％，２５～２６周的为２９．９２％。随离体时间延长，细胞活度逐渐下降，在保存温度为３７℃时更明显。笔者建议，做胎脑皮质组织及细胞移植时，供体胎龄以１６～２０周为宜，供体离开母体至移植的时间应控制在２小时内，愈早愈好，胎脑保存温度以４℃为宜。Cortical cell suspension was got from 12 cases of fetal cadaver in 16￣26 week gestation by mechanical method. The Trypan blue exclusive and fluorescein diacetate uptake tests were used to examine the viability. The viability at the first hour after isolation was 48.38% in the donors of 16￣20 week gestation, 38. 71% in those of 20￣24 week gestation and 29.92% in 24￣26 week gestation. The preservation prolonged, the viabilities descended gradually. The viability at 4℃ was higher than that at 37℃. The results showed that: the better donor age was 16￣20 weeks; the duration of preservation should be limited within two hours. the earlier the better; 4℃ was suitable for cortical tissue preservation.

关 键 词：胎脑移植 细胞活度 二乙酸荧光素 体外 

分 类 号：R651.11[医药卫生—外科学]