小脑共济失调大鼠胎脑移植后氨基丁酸及环磷酸鸟苷的改变  

GABA, cAMP and cGMP changes after fetal cerebellar transplantation in rats with ataxia

作　　者：陆钦池[1] 蔡琰[1] 朱华[1] 苏敏[1] 孙晓江[1] 

机构地区：[1]上海第二医科大学附属仁济医院神经内科

出　　处：《中华器官移植杂志》1994年第3期100-102,共3页Chinese Journal of Organ Transplantation

基　　金：上海市高等教育局资助

摘　　要：应用立体定位技术将兴奋性神经毒素海人藻酸直接注入大鼠小脑内造成小脑性共济失调的动物模型，用妊娠第１４～１６天的胚胎大鼠小脑制成细胞悬液移植至模型动物的小脑内。术后３个月处死动物，用氨基酸自动分析仪测定小脑内的主要抑制性递质氨基丁酸（ＧＡＢＡ）和脑内其它主要氨基酸，用放射免疫方法测定小脑内的环磷酸腺苷（ｃＡＭＰ）、环磷酸鸟苷（ｃＧＭＰ）含量。结果胚胎脑组织移植可使下降的ｃＧＭＰ上升，使升高的ＧＡＢＡ下降。Cerebellar ataxia model was produced by direct stereotactic injection of kainic acid (a neuroexcitotoxin) into the rat cerebellum. After an interval of 2￣3 weeks, the embryonic (14￣16 days of gestation) cerebellar suspension was transplanted into the cerebellar region of the models. The animals were sacrificed after three months. GABA and other amino acid, cAMP and cGMP were measured. The results showed that the fetal cerebellum transplantation could make the decreased cGMP increase and increased GABA decrease.

关 键 词：小脑共济失调 氨基丁酸 胎脑移植 

分 类 号：R651.11[医药卫生—外科学]