新城疫病毒在鸡胚中增殖影响因素探讨  

Studies on several factors affecting the hemagglutinin titer of NDV

作　　者：李京培[1] 毕克菊[1] 苏世云[1] 胡勇[1] 方海红[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物教研室,合肥230032

出　　处：《安徽医科大学学报》2005年第3期222-225,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的探讨新城疫病毒(NDV)Ⅱ系弱毒株在鸡胚内增殖产生血凝素的最佳条件。方法将NDV按一定浓度稀释接种到鸡胚内孵育48～72h,收获鸡胚尿囊液,测定血凝效价。结果选择9～11日龄鸡胚,种子病毒稀释10-2～10-3、接种剂量为0.2～0.3ml;在36℃培养48～72h时,其鸡胚尿囊液量多且血凝效价高。弱毒系产生的NDV血凝效价高于强毒系和C30克隆系病毒。鸡胚培养较鸡成纤维细胞培养产生的NDV血凝效价高。结论鸡胚的病毒接种量和孵育温度对NDV的血凝效价的影响很大,在研究NDV的血凝能力时应注意到这些问题。Objective To discuss the best condition in which NDVⅡ prodiferates in chicken embryo and produce hemagglutinin. Methods The NDV is inoculated into chicken embryo and hatched for 48 ~72 h. After the allantoid fuild of the chicken embryo had been reaped,HA titer was measuraed.Results The fallowing factors were found to be im portant in obtaining high titer hemagglutinin: ① 0.2~0.3 ml of the virus(10~ (-1)～10^(-7)) was inoculated into 8～10 d chicken embryo and cultured f or 48～72 h at 36℃. ② The HA titer in allantoid fuild of chicken embryo inocul ated with diluted virus was higher than that in the embryo inoculated with undil uted virus. ③ The HA of NDV produced by weak toxin NDV were higher than that were produced by strong toxin virus NDV or C_(30) clone. ④ The HA of NDV fos tered by chicken embryo were higher than that fostered by chicken fibrin cells. Conclusion The dosage of inoculating virus and the temperature of fostering have prominent effect in the HA titer of NDV. We must pay attenti on to these while investgating the hemagglutinin effect of the NDV.

关 键 词：血凝素类 新城疫病毒 

分 类 号：R373.9[医药卫生—病原生物学] R392.11[医药卫生—基础医学]