中药双黄补对人牙周膜细胞总蛋白含量和超微结构的影响  

Effects of Shuanghuangbu on the total protein content and ultrastructure of cultured human periodontal ligament cells

作　　者：许彦枝[1] 邹慧儒[1] 王小玲[1] 刘世正[1] 王永军[1] 

机构地区：[1]石家庄河北医科大学第四医院口腔科,050011

出　　处：《实用口腔医学杂志》2005年第3期374-377,共4页Journal of Practical Stomatology

基　　金：河北省科技厅资助课题(02276103D-19)

摘　　要：目的:探讨中药双黄补对人牙周膜细胞总蛋白含量和超微结构的影响。方法:采用组织块法体外培养人牙周膜细胞,取第5代培养细胞随机分为实验组和对照组,实验组加不同浓度的双黄补,对照组只加培养液。通过考马斯亮蓝法测定牙周膜细胞总蛋白含量,透射电子显微镜观察细胞超微结构的变化。结果:不同浓度的中药双黄补对人牙周膜细胞的总蛋白含量均有提高作用,其中尤以100μg/ml效果最佳。电子显微镜下可见胞质内粗面内质网、线粒体等细胞器数量增多。结论:中药双黄补可促进人牙周膜细胞的蛋白质合成,使细胞代谢更旺盛,功能更活跃。Objective:To observe the effects of Shuanghuangbu on the to tal protein content and ultrastructure of human periodontal ligament cells(PDLCs ).Methods:Human PDLCs of the 5th passage were treated with Shua nghuangbu(μg/ml) at 10,50,100,500 and 1 000 respectively for 120 h.Commasie bri lliant blue staining was used to study the total protein content and transmissio n electron microscopy was used to study the ultrastructure of PDLCs.Resu lts:The total protein content of human PDLCs increased after exposure of the cells to Shuanghuangbu at 10～ 1 000 μg/ml(P<0.05, vs control),the drug at 100 μg/ml showed the most potent effect. Under the transmission electro n microscope,there were more rough endoplasmic reticulums and mitochodrias in th e experimental group than in the control.Conclusions:Shuanghuang bu may stimulate the protein synthesis and metabolism of human PDLCs.

关 键 词：牙周膜细胞 细胞培养 中药 总蛋白含量 超微结构 

分 类 号：R285[医药卫生—中药学]