猴脑选择性超深低温断血流复苏对细胞因子影响的实验研究  

Effects of blood flow occlusion and resuscitation under selective monkey cerebral ultra-profound hypothermia on cytokines: an experimental study

作　　者：姜涛[1] 徐蔚[1] 江基尧[2] 

机构地区：[1]昆明医学院附属二院神经外科,昆明650031 [2]仁济医院神经外科,上海200003

出　　处：《中华神经医学杂志》2005年第6期568-571,共4页Chinese Journal of Neuromedicine

基　　金：云南省自然科学基金(2003C0010Z)

摘　　要：目的观察猴脑选择性超深低温断血流复苏实验前后血清中IL-2,IL-10,TNF-α,IFN-γ的动态变化,了解脑缺血后选择性超深低温复苏对猴免疫功能的影响。方法健康恒河猴10只,随机分为两血管阻断冷灌注组(4只),四血管阻断冷灌注组(4只)和常温灌注组(2只)。应用ELISA法统一测定实验前后血清中IL-2,IL-10,TNF-α,IFN-γ的浓度。结果两血管组4只恒河猴术后安全复苏并长期存活,四种细胞因子在灌注结束后较术前均明显增高(P<0.05),24h后恢复至术前水平,72h测得值与术前无显著差异(P>0.05)。常温组2只恒河猴及四血管组4只恒河猴于灌注后均未能安全复苏,全部死亡。其中四血管组与两血管组比较在灌注结束后IL-10,TNF-α增高更明显(P<0.05),IL-2,IFN-γ增高与两血管阻断冷灌注组比较无显著差异(P>0.05)。结论猴脑选择性超深低温断血流复苏可引起抗炎细胞因子与促炎细胞因子一过性增高,由于促炎因子与抑炎因子之间互相抑制,互相作用,两类因子同时增高可使细胞因子网络维持动态平衡,有利于机体免疫功能的重新建立。Objective To investigate the dynamic variations in serum interleukin-2(IL-2),interleukin-10(IL-10),tumor necrosis factor alpha(TNF-α),INF-γ in monkey of resuscitation after selective cerebral ultra-profound hypothermia and blood flow occlusion and study the effects of selective cerebral ultra-profound hypothermiac resuscitation after cerebral ischemia on immunological function in monkey. Methods 10 macaca rhesuses aged 4~10 years were randomized into 3 groups: two-vessel occlusion group(n=4),four-vessel occlusion group(n=4),and normothermia perfusion group(n=2). All the levels of these cytokines were measured by enzyme-linked immunosorbent assay(ELISA) before and after blood flow occlusion and resuscitation under selective ultra-profound hypothermia. Results Among all 10 monkeys,4 in the two-vessel occlusion group were successfully resuscitated and even lived for a long time. The levels of the 4 cytokines were significantly increased after perfusion (P<0.05),descended to pre-operational levels after 24 h,and resumed to the same levels as before operation after 72 h. All of the monkeys in normothermia perfusion group and four-vessel occlusion group were not resuscitated after perfusion and died. In the four-vessel occlusion group,the level of TNF-α after perfusion was more significantly increased than that in the two-vessel occlusion group(P<0.05). Conclusion The levels of pro-inflammatory and anti-inflammatory cytokines were significantly transiently increased after monkey’s resuscitation after selective cerebral ultra-profound hypothermia and blood flow occlusion. Because pro-inflammatory and anti-inflammatory cytokines affect each other,the simultaneous increase of 2 cytokines can maintain the dynamic equilibrium of cytokine network,which is advantageous for reconstruction of immunological function.

关 键 词：超深低温 断血流 实验研究 猴脑 IL-10 TNF-α IFN-γ IL-2 ELISA法 促炎细胞因子 抗炎细胞因子 细胞因子网络 机体免疫功能 四血管阻断 一过性增高 恒河猴 冷灌注 动态变化 低温复苏 长期存活 抑炎因子 促炎因子 互相作用 

分 类 号：R651[医药卫生—外科学]