银杏黄酮甙对糖尿病大鼠肾组织病理变化的影响  

The Effects of Ginkgo Flavonol on the Pathologic Changes of Kidney in Diabetic Rats

作　　者：侯淑芬[1] 张海松[1] 刘占全[1] 李会敏[1] 梁淑新[1] 

机构地区：[1]河北职工医学院附属医院肾内科,保北保定071000

出　　处：《中国误诊学杂志》2005年第6期1012-1015,共4页Chinese Journal of Misdiagnostics

摘　　要：目的:观察银杏黄酮甙对糖尿病大鼠肾组织MMPs-3、PAI-1表达及肾组织病理变化的影响,探讨其对糖尿病大鼠肾脏保护作用。方法:选择雄性SD大鼠48只,随机分为3组,即正常组(N组),糖尿病组(DM组),银杏黄酮甙治疗组(C组)。DM组、C组腹腔注射链脲佐菌素(STZ)制模成功后,C组给予银杏黄酮甙灌胃,分别于6、10周各组随机抽取8只宰杀,留肾组织标本用于做病理及免疫组化检查,所有数据以均数±标准差表示,用SPSS10.0统计软件处理,组间比较用方差分析。结果:免疫组化:第6周时,DM组、C组PAI-1表达均高于N组(统计学处理分别为P<0.01,P<0.05),至第10周时,C组PAI-1表达低于DM组(P<0.01)。第6周时,MMPs-3在DM组的表达低于N组(统计学处理P<0.05),持续至第10周,C组则高于N组、DM组(与两组比较P<0.01)。病理组织观察:6周时DM组、C组肾小球体积增大,电镜显示肾小球基底膜增厚,10周时DM组肾小球局部毛细血管袢玻璃样物质沉积,C组肾小球系膜区少量玻璃样物质的沉积,病变较DM组轻。结论:STZ诱导的糖尿病大鼠存在纤溶系统功能紊乱,细胞外基质积聚,PAI-1在肾组织表达增强,MMPs-3表达减弱,应用银杏黄酮甙干预治疗,可以使PAI-1的表达下调,MMPs-3的表达明显上调,使肾脏的病变减轻,其对糖尿病大鼠肾脏具有保护作用。Objective:To investigate the effects of Ginkgo Flavonol on the pathologic changes of kidney in diabetic rats,and to investigate its protective mechanism.Methods:48 male Sprague-Dawley rats were randomly divided into three groups:the control rats(Group N), the diabetic rats(Group DM)and the diabetics rats treated with Ginkgo Flavonol(Group C). The model of diabetic rats were made by peritoneal injection of Streptozotocin(STZ) in Group DM and Group C,then rats in Group C was treated with Ginkgo flavonol. 6 weeks and 10 weeks later 8 rats in each group were randomly sacrificed and immunohistochemical technique (SP assay) was performed to examine the expressions of matrix metalloproteinases-3(MMPs-3), Plasminogen activator inhibitor1(PAI-1) in renal cortex and the pathologic changes were observed,respectively. The experimental data was analyzed by SPSS 10.0.Result:The levels of renal PAI-1 were higher in Group DM?C than those in Group N(P<0.01,P<0.05).At 10th week,it was lower in Group C than in Group DM(P<0.05); The levels of renal MMPs-3 were lower in Group DM than in Group N(P<0.05),But in Group C higher than in Group N?DM(P<0.01).The pathologic changes at 6th week showed the glomerular volume increased in Group DM and C.the glomerular basement membrane rose under electronic microscope. At 10th week, glomerular capillary hyalinosis deposited in Group DM. And the pathologic changes in Group C were less than in Group DM.Conclusion:STZ-induced diabetic rats showed the strong expression of renal PAI-1 and the low expression of renal MMPs-3. Ginkgo Flavonol could down-regulate the expression of PAI-1,and up-regulate the expression of MMPs-3. Ginkgo Flavonol should play a part role in protecting the diabetic rats from nephropathies.

关 键 词：银杏 黄酮甙 药理学 糖尿病肾病 病理学 肾病理学 实验性 DN 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]