HE和Giemsa染色在结膜刮片检测嗜酸粒细胞的染色比较  

The Comparison of HE Staining and Giemsa Staining in Detection of Eosinophilic Granulocytes in Conjunctiva Scroping

作　　者：林健贤[1] 张平[1] 李永平[1] 郑健樑[1] 钟秀风[1] 张文忻[1] 聂莉[1] 张波[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科学报》2005年第2期79-81,共3页Eye Science

摘　　要：目的:通过HE和Giemsa对嗜酸粒细胞的染色效果比较,寻找最佳染色方法。方法:对春季结膜炎20例病人进行结膜刮片,并分别作HE和Giemsa染色,最后用光学普通显微镜观察。结果:在显微镜下,Giemsa染色的嗜酸粒细胞比HE染色的嗜酸粒细胞清晰。结论:实验表明,检测嗜酸粒细胞,采用Giemsa染色,可获得满意的染色效果。Purpose: To find the better method of eosinophilic granulocytes staining by comparison between HE staining and Giemsa staining Methods: Twenty conjunctivitis patients(40 eyes) were studied. The infected palpabral conjunctiva of both eyes were scroped and stained separately with Giemsa staining and HE staining. Result: Under microscope, eosinophilic granulocytes were more clear by Giemsa st-aining than HE staining Conclusion: In showing eosinophilic granulocytes, Giemsa staining was better than HE staining..

关 键 词：GIEMSA染色 嗜酸粒细胞 检测 刮片 春季结膜炎 效果比较 染色方法 微镜观察 显微镜下 HE染色 染色效果 

分 类 号：R446.1[医药卫生—诊断学]