赤芍治疗热毒血瘀证的血清蛋白质组变化的初步研究  

Preliminary Study on Change of Serum Proteome in Noxious Heat Blood Stasis Syndrome Treated by Radix Paeoniae Rubra

作　　者：谢文光[1] 马晓昌[2] 邵宁生[3] 丁勤学[3] 赵馨[3] 刘农乐[3] 魏钰书[1] 王会信[3] 陈可冀[2] 

机构地区：[1]川北医学院附属医院,四川南充637000 [2]中国中医研究院西苑医院 [3]军事医学科学院基础医学研究所

出　　处：《中国中西医结合杂志》2005年第6期520-524,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助(No.30271658)

摘　　要：目的研究赤芍对大鼠热毒血瘀证的血清蛋白质组变化的影响。方法用双向电泳(two-di-mensionalelectrophoresis,2DE)分析正常组、内毒素组〔用脂多糖(Lipopolysaccharide,LPS)静脉注射〕、赤芍组(灌胃给药)、LPS(静脉注射)+赤芍(灌胃给药)组之间的血清蛋白质组表达差异。结果(1)LPS致热毒血瘀证大鼠的血清在2DE胶上13个蛋白点(xPr)出现非常明显的含量变化,与正常组比较,LPS组的xPr16、xPr19的点容量值均显著降低,xPr1、xPr2、xPr3、xPr4、xPr6、xPr7、xPr8、xPr9、xPr11、xPr12、xPr23的点容量值均显著增高。(2)LPS+赤芍组与LPS组比较,LPS引起增高的xPr1、xPr2、xPr3、xPr4、xPr9的点容量值均显著降低,LPS引起降低的xPr16的点容量值显著增高,但xPr16的点容量值仍显著低于正常组,xPr1、xPr4、xPr9的点容量值均显著高于正常组;LPS+赤芍组的xPr2、xPr3两个蛋白点已被明显调节到正常状态;赤芍组除xPr2、xPr4外,在xPr1,xPr3,xPr9,xPr16点均表现对LPS显著的交互作用。(3)对xPr19,赤芍和LPS两因素显著的交互作用有协同性。(4)赤芍组xPr13、xPr14的点容量值均显著高于正常组,xPr15、xPr17的点容量值均显著低于正常组,这4个蛋白点LPS组与正常组比较差异均无显著性。结论通过调节xPr1、xPr2、xPr3、xPr4、xPr9、xPr16蛋白点,可能是赤芍治疗热毒血瘀证的疗效的分子基础。ObjectiveTo study the effect of red peony root (RPR) on serum proteome in rat suffering from noxious heat with blood stasis Syndrome (NH-BS). MethodsThe differences of serum proteome among rats in four groups, treated with lipopolysaccharide(LPS), RPR, LPS+RPR and saline respectively, were analyzed by bi-dimensional electrophoresis (2DE) assay. LPS was administered by intravenous injection and RPR by oral intake. Results(1) Serum of rats with LPS induced NH-BS showed significant changes in volume of serum protein (xPr) in 13 points on 2DE collagen, the volume of xPr 16 and 19 were significantly lower, volume of xPr 1, 2, 3, 4, 6, 7, 8, 9, 11, 12 and 23 were significantly higher respectively, as compared with those in the normal control group. (2) After being treated with RPR, the increased volume of xPr 1, 2, 3, 4 and 9 significantly decreased, and the decreased xPr 16 significantly increased, with xPr 2, 3 restored to normal level but the xPr16 still lower and xPr 1, 4, 9 higher than those in the normal group. RPR showed interaction with LPS on xPr 1, 3, 9, and 16. (3) For xPr 19, the interaction of RPR with LPS might be synergistic. (4) In the group treated with RPR, volumes of xPr 13 and 14 were significantly higher and those of 15, 17 were significantly lower than those in the normal group respectively, but the similar changes didn't found in the LPS group.ConclusionThe molecular basis of therapeutic effect of RPR on NH-BS might be through the regulation of xPr1, 2, 3, 4, 9 and 16.

关 键 词：血清蛋白质组 热毒血瘀证 赤芍 治疗 步研究 静脉注射 灌胃给药 交互作用 LPS 正常组 双向电泳 表达差异 含量变化 正常状态 分子基础 量值 白点 脂多糖 内毒素 2DE 增高 协同性 显著性 大鼠 调节 

分 类 号：R255[医药卫生—中医内科学]