玉米叶绿素含量测定方法研究  

Determined Methods of Chlorophyll from Maize

作　　者：李得孝[1] 郭月霞[1] 员海燕[1] 张敏[1] 龚小艳[1] 穆芳[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《中国农学通报》2005年第6期153-155,共3页Chinese Agricultural Science Bulletin

基　　金：陕西省科技攻关项目(2003K02-G1-02);西北农林科技大学2003年青年科技专项基金(080807)

摘　　要：以玉米幼苗绿叶为试材,研究了6种叶绿素提取液的吸收光谱和提取效率及低温冷冻对叶绿素提取的影响,结果表明:6种叶绿素提取液的吸收光谱基本相似,可以沿用Arnon法的计算公式进行叶绿素含量的测定;室温下,浸提法要优于研磨法,混合液浸提叶绿素比单独用丙酮或乙醇浸提要快,其中丙酮乙醇(水)配伍提取速度较快而稳定,是最佳的叶绿素提取方法。叶片冷冻后快速浸提确实能加速叶绿素提取速度,但叶绿素易受光热破坏,故应注意遮光。The absorption spectrums and extraction efficiencies of chlorophyll from maize seedling after frozen treatment were compared on six determined methods using different organic solvents. The results showed that the absorption spectrums of five solutions were identical with that of acetone solution, so Arnon's formula of chlorophyll was suitable for other methods. In house temperature, soaking methods were better than Arnon method. The extraction efficiencies of chlorophyll using mixture solution better than using acetone or ethanol alone, methods of acetone mixed ethanol were preferable for fast extraction and stability. Soaking methods in 50℃ after frozen treatment could accelerate extraction of chlorophyll, but also speed up degradation of chlorophyll, so light screening was necessary.

关 键 词：含量测定方法 吸收光谱 叶绿素含量 玉米幼苗 低温冷冻 提取效率 计算公式 提取方法 提取液 研磨法 浸提法 混合液 热破坏 乙醇 丙酮 速度 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O734[轻工技术与工程—食品科学与工程]