大叶黄杨无芽茎段不定芽再生及其起源  

Regenerating and Origin of Adventitious Shoots from Inter-Node Explants of Euonymus japonicus in vitro

作　　者：王茂良[1] 任桂芳[2] 王建红[2] 冯慧[2] 赵梁军[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094 [2]北京市园林科学研究所,北京100102

出　　处：《林业科学》2005年第3期179-181,i001,共4页Scientia Silvae Sinicae

基　　金：北京市科技计划项目 (H0 2 0 6 2 0 1 1 0 1 30 )

摘　　要：In order to improve the resistance of Euonymus japonicus , its ability of adventitious shoots regenerating from inter_node stem_segment were researched on MS medium in vitro . The effect of both plant growth regulators and carbon resources in media on adventitious shoots differentiating was studied. Regenerating system of E. japonicus was established successfully. Adventitious shoots were highly obtained from inter_node explants inoculated on MS media supplemented with 6_BA 1 7 mg\5L -1 + IBA 0 005 mg\5L -1 or 6_BA 1 9 mg\5L -1 + IBA 0 03 mg\5L -1 . The regenerating ratio reached to 52 4% and 46 7% respectively. Media for inducing adventitious roots were 1/2MS+ IAA 0 5 mg·L -1 . Adventitious shoots originated from surface tissue of inter_node stem_segment.In order to improve the resistance of Euonymus japonicus , its ability of adventitious shoots regenerating from inter_node stem_segment were researched on MS medium in vitro . The effect of both plant growth regulators and carbon resources in media on adventitious shoots differentiating was studied. Regenerating system of E. japonicus was established successfully. Adventitious shoots were highly obtained from inter_node explants inoculated on MS media supplemented with 6_BA 1 7 mg\5L -1 + IBA 0 005 mg\5L -1 or 6_BA 1 9 mg\5L -1 + IBA 0 03 mg\5L -1 . The regenerating ratio reached to 52 4% and 46 7% respectively. Media for inducing adventitious roots were 1/2MS+ IAA 0 5 mg·L -1 . Adventitious shoots originated from surface tissue of inter_node stem_segment.

关 键 词：大叶黄杨 无芽茎段 不定芽诱导 不定芽起源 

分 类 号：S722.37[农业科学—林木遗传育种] S718.46[农业科学—林学]