肾小球系膜细胞产生的白介素1及前列腺素E_2对该细胞增殖的自身调节  

SELF— REGULATION OF MESANGIAL CELLS (MsC) PROLIFERATION BY MsC — DERIVED

作　　者：谌贻璞[1] 高进[1] 赵志辉[1] 李丽英[1] 王海燕[1] 

机构地区：[1]北京医科大学肾病研究所,100034

出　　处：《中华肾脏病杂志》1994年第4期237-238,共2页Chinese Journal of Nephrology

基　　金：国家自然科学基金 3870732

摘　　要：已知白介素1(IL_1)可引起巨噬细胞释放前列腺素E_2(PGE_2),而PGE_2反之可拮抗巨噬细胞产生的IL_1对胸腺细胞增殖的刺激作用。为检验肾小球系膜细胞(MC)是否也有类似效应。特作本文试验。方法与结果选用首代亚培养的大鼠MC进行试验。一、脂多糖(LPS)刺激MC产生IL_1:用LPS与MC共同卵育48h后收集上清,以鼠胸腺细胞增殖试验(n=4)检测IL_1活性。结果如表1。二、MC-IL_1刺激MC产生PGE_2:用上面获得的含系膜细胞白介素1(MC-IL_1)的上清50μl加Hanks液450μl,与MC孵育2h,然后收集上清用放射免疫试验(n=4)检测PGE_2。结果如表2。用外源性纯IL_1(100u/This study was designed to investigate whether there is an antagonistic effect of MsC—derived IL—1 and PGE2 on MsC proliferation. Experimental results showed that: (1)MsC stimulated by LPS could produce IL—1; (2) MsC stimulated by MsC—derived IL—1 could release PGE2; (3) exogenous PGE2(100ug/ml) inhibited the MsC proliferation induced by MsC—derived IL—1; (4) indomethacin(2ug/ml) which inhibited the production of endogenous PGE2, enhanced the MsC proliferation induced by MsC—derived IL—1. The results suggest that there is a self—regulation effect on MsC proliferation by MsC—derived IL—1 and PGE2. Clinically, the beneficial effect of PGE2 should be protected, so that the abuse of indomethacin in the treatment of glomerulonephritis should be avoided.

关 键 词：肾小球 膜细胞 白细胞介素1 

分 类 号：R692.02[医药卫生—泌尿科学]