用ELISA捕获法测抗甲型肝炎病毒IgG及与竞争法测抗甲型肝炎病毒的比较  

Detection with Capture ELISA of Anti-HAV IgG and Comparision with Contest ELISA of Anti-HAV

作　　者：周志统[1] 夏绍源[1] 吴昌烈[1] 谢爱珑 奚中华 郑志清[1] 

机构地区：[1]上海市传染病医院,上海市儿童医院

出　　处：《中华实验和临床病毒学杂志》1994年第1期55-57,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：用抗甲型肝炎病毒（ＨＡＶ）ＩｇＧ阴性血清处理以抑制非特异反应，提高灵敏性的捕获法酶联免疫吸附试验检测甲型肝炎病毒特异ＩｇＧ抗体，并与竞争法检测总抗ＨＡＶ进行了比较。结果为甲型肝炎患者发病早期血清１：２０稀释时，抗ＨＡＶＩｇＧ２８／３０例阳性，总抗ＨＡＶ３０／３０例阳性。血清１：１０００稀释时，则抗ＨＡＶＩｇＧ为８／３０例阳性，总抗ＨＡＶ２９／３０例阳性。若以此稀释度为诊断界限，８例抗ＨＡＶＩｇＧ阳性出现于发病第１０至１４天。实验提示甲型肝炎患者发病早期已产生抗ＨＡＶＩｇＧ，而仅测总抗ＨＡＶ是不能区别抗体类型的。A r-capture ELISA for detecting IgG antibody to Hepatitis A virus (HAV) has been developed and compared with contest ELISA of anti-HAV. It is useful that anti-HAVIgG negative sera inhibit nonspecific reaction in the capture ELISA of anti-HAV IgG. Anti-HAVIgG and anti-HAV were detected in 30 sera from patients with anti-HAVIgG positive. In acute stage, the titer of anti-HAVIgG was ≥1: 20 in 28 of 30 patients, but that of anti-HAV was ≥ 1: 20 in all patients. With diluted sera in 1:1000, 8 of 30 patients were anti-HAV positive. Five of 8 anti-HAVIgG ≥1: 1000 positive cases were detected after 14 days of illness and the remaining 3 were detected on the 10th day of illness. These results suggested that most of the patients with HAV infection have produced anti-HAVIgG, and anti-HAVIgG is only a part of anti-HAV in the early stage of the illness.

关 键 词：甲型肝炎病毒 ELISA 免疫球蛋白G 

分 类 号：R373.21[医药卫生—病原生物学]