检测丁型肝炎病毒RNA打点杂交法的建立及其应用  

Establishment and Application of Dot Hybridization for the Detection of (HDA-RNA in Serum Samples)

作　　者：易炎杰[1] 汤少华[1] 詹美云[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《中华实验和临床病毒学杂志》1994年第1期65-67,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：经缺口平移法以ａ－３２Ｐ－ｄＣＴＰ标记１．０ｋｂ的丁型肝炎病毒（ＨＤＶ）ｃＤＮＡ片段为探针，采用蛋白酶Ｋ直接从血清中提取ＨＤＶＲＮＡ，建立了检测血清中ＨＤＶＲＮＡ的打点杂交法，其灵敏性可达１ｐｇ水平，与乙型肝炎病毒（ＨＢＶ）ＤＮＡ无交叉杂交反应；并应用于检测我国５９４９份ＨＢｓＡｇ阳性血清中的ＨＤＶＲＮＡ，共检出１７６份ＨＤＶＲＮＡ阳性，检出率为２．９５％。A method for detecting HDV-RNA in serum samples has been developed by dot hybridization cDNA-RNA with 32p-labelled 1kb HDV cDNA probe. 1pg HDV cDNA was detectable and there was no cross-hybridization with HBV DNA. Five-thousand nine-hundred and forty-nine serum samples positive for HBsAg were tested by this assay and HDV-RNA was detected in 2.9% of these samples. This assay is a sensitive method to measure HD Viremia.

关 键 词：丁型肝炎病毒 打点杂交 RNA 检测 

分 类 号：R373.21[医药卫生—病原生物学]