乙型肝炎病毒核心基因的酶切图谱及核苷酸序列分析  

Studies on the Core Gene of Hepatitis B Virus by Restriction Enzyme Mapping and Sequence Analysis

作　　者：张蕾青[1] 何丽芳[1] 卫清[1] 胡德昌[1] 闻玉梅[1] 

机构地区：[1]上海医科大学医学分子病毒学实验室,上海医科大学中山医院消化科,贵州遵义医学院微生物教研室

出　　处：《中华实验和临床病毒学杂志》1994年第1期7-10,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家教委博士点基金

摘　　要：对慢性乙型肝炎患者的２２份肝穿刺活检组织及其中配对的６份血清用ＰＣＲ扩增出乙型肝炎病毒（ＨＢＶ）的ＰｒｅＣ／Ｃ基因，选用ＡｖａⅡ、Ｓａｕ３ＡⅠ、ＸｍｎＩ，ＢｓｔＮＩ及ＴａｑＩ５种限制性内切酶消化后，对Ｃ基因进行酶谱分析。发现有６份肝组织及１份血清出现异常酶谱。对Ｓａｕ３ＡⅠ酶解异常标本进行分子克隆及核苷酸序列分析，发现２例患者因２１３７位核苷酸点突变出现了Ｓａｕ３ＡⅠ的新酶切点。类似变化在肝癌组织的ＨＢＶＣ基因中也曾发现，其意义待进一步研究。Twenty-two liver biopsies and 6 serum samples from 22 chronic hepatitis B patients were used for PCR amplification of the precore and core gene. The following restriction enzymes were used to analyze the enzyme mapping of the core gene: Ava Ⅱ,Sau3AⅠ, Xmn I, Bst NI, Taq I. There were 6 liver tissues and 1 serum sample that showed enzyme mapping different from that of the adr subtype prototype (Chinese isolate pADR-1) used as a standard. Excluding the emzyme mapping which may be due to variations between different subtypes, 2 lives biopsy tissues showed point mutations at ucleotide 2137, which resulted in a new Sau 3AⅠsite. Similar mutation was also seen in the core gene isolated from hepatocellular carcinoma tissues. The significance of this point mutation merits further study.

关 键 词：乙型肝炎病毒 核心基因 酶切图谱 

分 类 号：R373.21[医药卫生—病原生物学]