登革热患者血液中病毒滴度的动态观察  

Dynamic Observations on the Virus-Carrying State of Culex Fatigans Experimentally Infected with Dengue Virus

作　　者：陈文洲[1] 陈玉本[1] 邝继深[1] 王飞[1] 王祖扣 陈明勇 谢卓布 王九生 

机构地区：[1]海南省卫生防疫站,海南省临高县卫生防疫站

出　　处：《中华实验和临床病毒学杂志》1994年第1期26-27,共2页Chinese Journal of Experimental and Clinical Virology

摘　　要：１９８６年海南岛Ⅱ型登革病毒流行时，对７例患者发病当天至第５天，定时取血观察血液中的病毒滴度变化情况。实验结果表明，病毒血症高峰期是在起病２天内（Ｌｏｇ１０ＴＣＩＤ５０／０．０２５ｍｌ为４．４４～≥８．５０，平均为７．３１）；病毒血症至少维持３天（Ｌｏｇ１０ＴＣＩＤ５０／０．０２５ｍｌ为２．００～８．３３，平均为４．７５）；第４天多数可（５／７）检出病毒（Ｌｏｇ１０ＴＣＩＤ５０／０．０２５ｍｌ为２．００～３．２３，平均为２．５０）；第５天病毒血症全部消失。提示，临床标本的采集时机，以及合理使用蚊帐，防止蚊子叮咬，对病原学诊断和控制登革热具有实际意义。This article reports observations on the virus-carrying state of Culex fatigans experimentally infected with dengue virus in November 1987. Adult Culexfatigans mosquitoes which had been capured from non-epidemic areas of dengue fever and ascertained to be noncarriers of dengue virus by examination were bred in the laboratory. The adult mosquitoes of their progeny were let to suck the blood of a patient with dengue fever within one day of the onset of the disease, meanwhile, venous blood was collected from this patient. The mosquitoes which had not sucked the blood were discarded. While those which had sucked enough blood were brought back to the room for mosquito breeding. These mosquitoes were chilled to death on the 29th day of infection and were emulsified. The emulsion of infected mosquitoes as well as the patient's serum were separately inoculated into Aedes albopictus C6 / 36 cell line and their virus infectivity titers were determined. Results showed that the virus infectivity titers in the patient's serum and that in the emulsion of infected mosquitoes were 5.50 and 2.33 logTCID50 / 0.025ml, respectively. Identification of the virus species carried out by the use of indirect immunofluorescence with each of four type-specific monoclonal antibodies against four corresponding types of dengue viurs confirmed that both the virus present in the patient's serum and that in the emulsion of infected mosquitoes were Type Ⅱ dengue virus which was identical to the type of dengue virus causing the epidemic of dengue fever at that time.Experimental results suggest that Culexfatigans is susceptible to dengue virus. Its period of dengue virus-carrying state after the infection was rather long and might persist for at least 29 days. Some virologists in China had carried out experimental studies on the transmission of dengue virus, they experimentally infected adult Culexfatigans mosquitoes which had been bred in the laboratory for a period and let these mosquitoes suck the blood of normal newborn mice. Blood samples tak

关 键 词：登革热 病毒血症 病毒滴度 

分 类 号：R512.801[医药卫生—内科学]