光敏生物素标记核酸探针在检测人巨细胞病毒感染中的初步应用  

Preliminary Application of Photobiotin-labelled Probe in the Detection of HCMV Infection

作　　者：丁健青[1] 徐桂林[1] 陈淑云[1] 季育华[1] 陆德源[1] 

机构地区：[1]上海第二医科大学微生物教研室

出　　处：《中华实验和临床病毒学杂志》1994年第2期146-148,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：探索建立了光敏生物素标记人巨细胞病毒（ＨＣＭＶ）ＤＮＡＨｉｎｄⅢＪ．Ｎ、Ｙ、Ｏ片段作为探针检测尿液中ＨＣＭＶＤＮＡ的分子杂交方法，检测灵敏度可达５ｐｇ。研究表明该法不仅有与同位素标记探针相同的特异性和相近的灵敏度，而且更具稳定、简便、安全等优点，利于在基层推广使用。This article explores a hybridization assay by using HCMV DNA Hind Ⅲ J, N, Y. O fragments as a probe labelled with photobiotin for the detection of HCMV DNA in urine samples. This probe was able to detect 5pg of homologous DNA. This study showed that the photobiotin-labelled HCMV DNA probe to detect HCMV DNA in urine samples not only had high specificity and sensitivity similar to that of isotope-labelled probe,but also had the advantages such as stability, simplicity and security, so it could be popularized and accepted by many general laboratories.

关 键 词：人巨细胞病毒 光敏生物素 感染 

分 类 号：R512.990.4[医药卫生—内科学]