人乳头瘤病毒16型E6E7ORF转化作用的研究  

Human Papillomavirus Type 16 E6E7 ORF Induced Malignant Transformation of NIH 3T3 Cells

作　　者：侯芷英 孙亚洲[1] 商铭 明利华[1] 

机构地区：[1]中国医学科学院肿瘤研究所

出　　处：《中华实验和临床病毒学杂志》1994年第2期112-115,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：构建了含人乳头瘤病毒１６型（ＨＰＶ１６）－Ｅ６Ｅ７ＯＲＦｓ（ｎｔ８３－８５５）片段和ＨＰＶ１６长控制区（ＬＣＲ）加Ｅ６Ｅ７ＯＲＦｓ片段（ｎｔ７００７－７９０４／０－８７９）的逆转录病毒载体ｐＨ２１和ｐＨ１８质粒，利用Ｌｉｐｏｆｅｃｔｉｎ分别将它们导入病毒包装细胞ｐＡ３１７中，经过筛选获得Ｇ４１８抗性的病毒包装细胞，产生的重组病毒Ｈ２１和Ｈ１８感染的ＮＩＨ３Ｔ３细胞都具有恶性细胞的形态学特征，并能在裸鼠体内形成肿瘤。Ｓｏｕｔｈｅｒｎ杂交结果证明，上述两基因片段都整合到细胞基因组中。本实验结果说明ＨＰＶ１６－Ｅ６Ｅ７基因片段是ＨＰＶ１６转化ＮＩＨ３Ｔ３细胞的关键早期区，其自身ＬＣＲ区在该转化过程中没有显示出重要作用。A retrovirus vector containig human papillomavirus type 16 E6E7 ORFs(nt83-855)and another retrovirus vector containing human papillomavirus type 16 were packaged with helper cells PA317.NIH 3T3 cells had been transformed by the pseudotype retroviruses H21 and H18, respectiely. The transformed cells can cause tumor in nude mice and the tumors were diagnosed histologically as fibrosarcoma. These results show that the HPV16-E6E7 ORFs have the transforming potentiality in NIH3T3 cells and HPV16 own LCR region is not necessary in process of transforming NIH3T3 cells. The transformed cells provide new models for analyzing the transforming functions of HPV 16.

关 键 词：人乳头瘤病毒 16型 转化 逆转病毒 

分 类 号：R373.9[医药卫生—病原生物学]