补肾清热毒方对cGVHD狼疮小鼠肾组织细胞Fas的调节作用  

The Effects of BQR on Fas in Kidney of Lupus Nephritis Models

作　　者：任文英[1] 王新高[2] 陈香美[3] 邱全瑛[4] 吕扬[3] 洪权[3] 师锁柱[3] 

机构地区：[1]福建省人民医院,福州350004 [2]北京天坛医院,北京100050 [3]中国人民解放军总医院,北京100853 [4]北京中医药大学,北京100029

出　　处：《中国中西医结合肾病杂志》2005年第5期255-258,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：福建省中医药重点基金资助项目(No.702)

摘　　要：目的:观察补肾清热毒方(简称补清方)对cGVHD狼疮样小鼠模型肾组织细胞Fas的调节作用。方法:将模型小鼠于诱导后第4周,随机分为补清方组(10只),泼尼松组(10只),模型组(6只)及未造模的F1对照组(6只),用药8周后处死,采用免疫组化、Westernblot和RT-PCR技术检测Fas基因转录及其蛋白表达情况。结果:补清方可上调FasmRNA和蛋白水平。结论:补清方治疗LN的作用可能是通过上调Fas,促进细胞凋亡实现的。Objective:To study the effects of traditional Chinese medicine BQR on Fas in kidney of mice lupus nephritis models.Methods:Four weeks after induction of cGVHD lupus-like nephritis, 34 female mice were randomly divided into four groups: BQR group(10), prednisone group(10), model group(6) and control group(6). Mice were treated for 8 weeks before being killed. Immunohistochemistry and western blotting were used for detection of Fas protein. The transcription of Fas mRNA in kidney was examined by RT-PCR.Results:BQR increased the protein and mRNA level of Fas.Conclusion:BQR is effective for the treatment of lupus nephritis probably by facilitating Fas-mediated apoptosis in kidney.

关 键 词：补肾清热毒方 cGVHD狼疮 小鼠 肾组织细胞 FAS 狼疮性肾炎 细胞凋亡 

分 类 号：R285[医药卫生—中药学]