痢疾菌侵袭性质粒抗原（Ipa）及O抗原与侵入细胞能力的关系研究  

THE PATHOGENESIS OF SHIGELLASPECIES:ROLES OF INVASIVE OUTER MEMBRANE PROTEINS AND O ANTIGEN IN THE ENTRY PROCESS

作　　者：章容萍 陈恩临[1] 

机构地区：[1]天津医学院微生物学教研室,天津第二医学院微生物学教研室

出　　处：《中华微生物学和免疫学杂志》1994年第1期19-21,共3页Chinese Journal of Microbiology and Immunology

摘　　要：采用痢疾菌Ｉｐａ＋Ｏａｇ－，ＩｐａＯａｇ＋及Ｉｐａ＋Ｏａｇ＋等三种不同的菌株分别进行了毒力及毒力相关表型实验。结果发现，Ｉｐａ＋ＯａＧ－株Ｓ１Ｒ１０１／ｐＨＳ４１０８与Ｉｐａ＋Ｏａｇ＋株ＢＳ１６９／ｐＨＳ４１０８都有侵入ＨｅＬａ细胞的能力，而Ｉｐａ－Ｏａｇ＋株Ｔ３２则无侵入ＨｅＬａ细胞的能力，只表达多肽ａ、ｅ、ｄ的菌株Ｓ１Ｒ１０２／ｐＪＭ５６亦不能侵入ＨｅＬａ细胞。从而证明，侵袭性质粒抗原（Ｉｐａ）对于细菌侵入ＨｅＬａ细胞则是足够的，其中多肽ｂ起关键性作用，而Ｏ抗原并不参与侵入过程或不成为其必要的前提条件。he virulence and the virulence-associated phenotypes of Shigella species were investigated with Ipa+Oag-,Ipa+ Oag +,and Ipa-Oag+ strains,respectively.Our results showed that strains S1R101/pHS4108(Ip8+ Oag-)andBS169/pHS4108(Ipa+ Oag+)were capable of entering HeLa cells ;while S. sonnei strain S1R102/pJM56 whichcould only express a,c,and d and S.flexneri strain T32(Ipa-Oag+)were incapable of entering HeLa Cells.Wesuggest hereby that invasive plasmid polypeptides alone may be sufficient for the bacillus into the cells,and O antigen may not be involved or play little role,if any ,in this process.

关 键 词：痢疾杆菌 侵袭性 外膜蛋白 O抗原 

分 类 号：R378.25[医药卫生—病原生物学]