用CL法和微量MPO法观察人参皂甙对人PMN吞噬杀菌作用  

INVESTIGATION OF GINSENOSIDE ON HUMAN PMN PHAGOCYTOSIS BY CL AND MPO MICROMEASUREMENT

作　　者：张力平[1] 沈海中[1] 程松高[1] 

机构地区：[1]首都医科大学微生物学教研室

出　　处：《中华微生物学和免疫学杂志》1994年第2期121-123,共3页Chinese Journal of Microbiology and Immunology

基　　金：中国科学院自然科学基金

摘　　要：采用化学发光（ＣＬ）法和髓过氧化物酶（ＭＰＯ）微量测定法检测了人参茎叶皂甙对人多形核白细胞（ＰＭＮ）吞噬金黄色葡萄球菌的影响。实验结果表明，人参茎叶皂甙作用过的ＰＭＮ－ＣＬ反应（ＣＬ３０和Ｐｅａｋ）和ＭＰＯ释放水平均增强，对ＣＬ３０与ＣＦＵ、ＭＰＯ与ＣＦＵ行直线相关分析均呈密切相关（ｒ＝０．９５８，ｐ＜０．００１；ｒ＝０．８７５，ｐ＜０．０１）。本实验提示，ＰＭＮ－ＣＬ法和ＭＰＯ微量测定法是研究药物对ＰＭＮ吞噬杀菌功能影响的客观、定量和快速的方法。The effect of ginsenoside on human polymorphonuclear leukocytte(PMN)phagocytosis and killing function wasevaluated by chemiluminescence(CL ) assay and myeloperoxidase(MPO )release micromeasurement. The resultsshowed that the CL response(CL30 and Peak )and MPO release level of ginsenoside-pretreated PMN were en-hanced.The CL30 was closely related to CFU of remainder S.aureus(r=0.875,P<0.01),the MPO release levelwas also closely related to CFU r=0.958,P<0.001),The experiment indicated that the CL assay and MPO re-lease micromeasurement were the quantitative ,objective and rapid method for evaluating the phagocytosis andkilling function of PMN effected by the medicines .

关 键 词：化学发光 人参茎叶 皂甙 PMN MPO 

分 类 号：R446.5[医药卫生—诊断学]