我国登革病毒分离株NS1基因的扩增  

AMPLIFICATION OF NS1 GENE OF THE HAINAN STRAIN OF DENGUE 2 VIRUS

作　　者：秦鄂德[1] 于曼[1] 杨佩英[1] 韩照中[1] 司炳银[1] 徐品芳 阎国珍[1] 

机构地区：[1]北京军事医学科学院微生物流行病研究所

出　　处：《中华微生物学和免疫学杂志》1994年第2期93-95,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金

摘　　要：本研究利用逆转录（ＲＴ－ＰＣＲ）技术将由聚乙二醇浓缩的登革病毒制备的ＲＮＡ合成为双股ｃＤＮＡ，建立了扩增登革病毒大片段ｃＤＮＡ的条件。采用该方法扩增了我国登革２型病毒株的ｃＤＮＡ片段，长度为１３８３ｂｐ。它不但包括完整ＮＳ１基因，而且也包括了对ＮＳ１编码蛋白的功能起重要作用的位于其上游和下游的部分碱基序列。登革病毒大片段ｃＤＮＡ的扩增有助于对我国毒株ＮＳ１基因的克隆与表达的研究。The genomic RNA of the dengue virus concentrated by polyethylene glycol was reversely transcriped and theresulting cDNA was amplified by using the RT-PCR. The optimal reaction conditions for amplifying large cDN Afragment of dengue virus were established. The amplified cDNA fragment of Hainan strain was about 1380 bp inlength.It included not only the whole NS1 gene,but also the partial base sequences located at upstream and down-stream of the NS 1 gene which can play an important role on the function of the protein expressed by NS1 gene.This cDNA fragment could be inserted into a plasmid for expression studies.

关 键 词：聚合酶链反应 NS1基因 登革病毒 

分 类 号：R373.33[医药卫生—病原生物学]