7株小鼠胸腺基质细胞系诱导裸鼠骨髓干细胞表达CD4及CD8分子  

SEVEN MTSC LINES INDUCE NUDE MICE BONE MARROW CELLS EXPRESSING CD4 CD8 MOLECULES

作　　者：张平夏[1] 陈慰峰[1] 陶家平[1] 

机构地区：[1]北京医科大学

出　　处：《中华微生物学和免疫学杂志》1994年第3期160-163,共4页Chinese Journal of Microbiology and Immunology

基　　金：美国洛氏中华医学基金(89-524);国家自然科学基金

摘　　要：为研究胸腺微环境在Ｔ细胞发育中的作用，我室在体外建立了７株小鼠胸腺基质细胞系，命名为ＭＴＥＣＩ～ＭＴＥＣ７。通过初步分离得到裸鼠骨髓富含干细胞的细胞群，表面ＣＤ４及ＣＤ８分子均为阴性。将分离的骨髓干细胞与胸腺基质细胞共育３天后，经双色荧光抗体染色，ＦＡＣＳ分析发现胸腺基质细胞可诱导裸鼠骨髓干细胞表达ＣＤ４ＣＤ８分子。ＭＴＳＣ－ＳＮ及ＭＴＳＣ主要诱导的是ＣＤ４＋ＣＤ８－细胞，部分ＣＤ４＋ＣＤ８＋细胞，而ＣＤ４－ＣＤ８＋细胞极少，这种诱导特点可能和基质细胞系的类型有关。MTSC1- 7cell lines, established in our laboratory,derived from thymus of BALB/cmice. These MTSC linesconstitutively produce multiple cytokines. T0 study the roles of MTSC lines on the differentiation of bone marrowcells,we cocultured MTSC 1- 7cells or MTSC 1- 7supernatants with nude mice separated fresh BM cells. The phe-notypes of viable BM cells after cocultured with MTSC cells or MTSC-SNs for 3 days, stained by PE-anti- L3T 4McAb and FITC-anti-Lyt2 McAb ,were analysed on flow cytometry (FACS ).The results showed that MTSC andMTSC-SN could induce BM cells expressing CD4 and CD8 molecules. The rates of these cells were CD4+CD8-11%- 39%,CD4+CD8+3%- 25%and CD4- CD8+0.9%-8%.These results suggest that the microenviroment whichallows T cell progenitors to generate CD4+CD8- cell differ from that yielding CD4+CD8+ and CD4-CD8+ cells.

关 键 词：胸腺基质细胞 骨髓干细胞 CD4 CD8 

分 类 号：R392.12[医药卫生—免疫学]