几种中药提取成分对人PMN吞噬杀菌功能的影响  

THE EFFECTS OF SEVERAL EXTRACTIVES OF TRADITIONAL CHINESE PHARMACEUTICALS ON HUMAN POL YMORPHONUCLEAR LEUKOCUTES PHAGOCYTIC AND KILLING FUNCTION

作　　者：张力平[1] 沈海中[1] 程松高[1] 

机构地区：[1]首都医科大学微生物学教研室

出　　处：《中华微生物学和免疫学杂志》1994年第4期277-279,共3页Chinese Journal of Microbiology and Immunology

基　　金：中国科学院自然科学基金

摘　　要：用人参茎叶皂甙、黄芪多糖、云芝胞内多糖、猪苓多糖及阿魏酸钠预先处理分离的人ＰＭＮ，测定ＰＭＮ吞噬杀伤金黄色葡萄球菌时的ＣＬ值（３０分种积分值和峰值，ＣＬ３０及Ｐｅａｋ）和ＭＰＯ水平。结果表明，人参茎叶皂甙、黄芪多糖、云芝胞内多糖和猪苓多糖在一定浓度范围内对人ＰＭＮ的ＣＬ和ＭＰＯ水平均有明显增强作用（Ｐ＜０．０１），而阿魏酸钠则抑制ＰＭＮ的ＣＬ和ＭＰＯ水平（Ｐ＜０．０１）。这表明人参茎叶皂甙、云芝胞内多糖、猪苓多糖和黄芪多糖均能增强人ＰＭＮ的吞噬杀菌能力，阿魏酸钠则抑制人ＰＭＮ的吞噬杀菌能力。While the respiratory burst occurs during the process of phagocytosis and bacteria-killing of polymorphonu-cleus leucocytes(PMN),the chemiluminescence(CL )appears.The luminol enhanced CL response is related withthe release of myeloperoxidase (MPO ).The separated PMN were pretreated separately with ginsenoside,astra-galu-polysaccharide (APS ),krestin polysaccharide(KPS ),polyporus umbellatus or sodium ferulate, then we ob-served PMN-CL response(CL30 and peak )and MPO upon the stimulation with S. aureus. Enhancement of both CLand MPO occured when the PMN were pretreated with ginsenoside,APS ,KPS ,polyporus umbellatus (P <0.01 ).Inhibition of both CL and MPO occured when the PMN were pretreated with sodium ferulate(P<0.01 ). Thesefindings indicated that ginsenoside,APS,KPS,polyporus umbellatus enhanced the phagocytosis and bacteria-killingfunction of human PMN, but sodium ferulate reduced the human PMN function.Either enhancement or reductionwas associated wih the MPO level of human PMN.

关 键 词：中药 提取成分 多形核白细胞 CL 

分 类 号：R285[医药卫生—中药学]