霍乱弧菌O139国内外菌株分子生物学特征比较  

COMPARISONS ON THE MOLECULAR CHARACTERISTICS OF VIBRIO CHOLERAE O139 ISOLATED FROM CHINA,ONDIA AND BANGLADESH

作　　者：段广才[1] 高守一[1] 祁国明[1] 刘延清[1] 刘彩莲[1] 徐文斌[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所

出　　处：《中华微生物学和免疫学杂志》1994年第6期373-375,共3页Chinese Journal of Microbiology and Immunology

摘　　要：１９９２年１０月在印度，１２月开始在孟加拉国发生了一种新型霍乱弧菌——霍乱弧菌Ｏ１３９引起的霍乱样大流行。我国新疆南部柯坪县于１９９３年５～６月也发生了由该型菌引起的典型霍乱样腹泻暴发。分子生物学研究表明：细菌染色体ＤＮＡ经核酸限制性内切酶ＳａｌⅠ消化后，用核酸脉冲场凝胶电泳（ＰＦＧＥ）分离ＤＮＡ，所有Ｏ１３９菌株表现为一致的特征性的限制性酶谱。用１６ＳｒＲＮＡ基因为探针，染色体ＤＮＡ经ＢｇｌⅠ酶切ＰＦＧＥ分离后做ｓｏｕｔｈｅｒｎｂｌｏｔ杂交分析，结果印度、孟加拉国和中国分离的菌株分别属于不同的核糖体基因型（Ｒｉｂｏｔｙｐｅ，ＲＴ），但与新疆菌株相比，印度和孟加拉国菌株的关系更为密切。结果还显示：霍乱弧菌Ｏ１３９菌株具有基本一致的基因组特征。Since October of 1992,large epidemics of cholera-like diarrhoea,caused by the new type of Vibrio cholerae Vibrio cholerae O139,have occcured in India and Bangladesh. In May to June of 1993,an outbreak caused by O139 strains occured in Keping,Xinjiang Autonomous Region of China.Molecular biological study showed:The restric-tion patterns of chromosomal DNAs of the strains detected were same and identical compared with other types of Vibrio cholerae after the chromosomal DNAs were digested with SalI and separated by pulsed-field gel elec-trophoresis (PFGE).By Southern blot hybridization analysis probed for 16S rRNA genes, after the chromosomal DNAs being digested with BglI and separated by PFGE, the strains isolated from India, Bangladesh and China be-longed to different ribotypes(RT).And the strains of India and Bangladesh had a close relationship compared with those isolated from China.

关 键 词：霍乱弧菌 O139 分子生物学 分子流行病学 PFGE 

分 类 号：R378.3[医药卫生—病原生物学]