霍乱弧菌O139某些生物学特性及毒力基因检测  

STUDY ON THE BIOLOGICAL CHARACTERISTICS OF VIBRIO CHOLERAE O139 ANDITS VIRULENCE GENES

作　　者：高守一[1] 段广才[1] 祁国明[1] 刘捷[1] 刘延清[1] 刘彩莲[1] 章丽娟[1] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,新疆维吾尔自治区卫生防疫站

出　　处：《中华微生物学和免疫学杂志》1994年第6期365-368,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金

摘　　要：Ｏ１３９霍乱弧菌是１９９２年发现的新型霍乱弧菌，其毒力强，危害严重。对中国、印度、孟加拉国分离的２３株Ｏ１３９菌株的部分生物学特征检查表明：对弧菌抑制剂Ｏ／１２９均具有抗性、溶原菌、山梨醇慢发酵、非溶血性。核酸分子杂交显示，所有被检Ｏ１３９菌株都具有主要的霍乱弧菌毒素基因：ｃｔｘ，ｚｏｔ，ａｃｅ和ＲＳ１序列。ＰＣＲ检测ｃｔｘ基因与Ｏ１群流行珠具有相同的扩增产物，ｔｃｐＡ基因扩增表现为与埃尔托型霍乱弧菌相似的扩增产物。兔肠段结扎测毒表明，Ｏ１３９霍乱弧菌为强毒株。因此Ｏ１３９菌与Ｏ１群霍乱弧菌流行株具有共同的毒力特征。Vibrio cholerae O139 is a new type of Vibrio cholerae found in 1992. In May of 1993,an outbreak of cholera-likediarrhoea caused by O139 occurred in Xinjiang of China. In this study,we characterized the strains isolated fromChina, India and Bangladesh with biological and toxigenic gene probing techniques.The results demonstrated thatall strains of O139 were lysogenic, sorbitol slow fermentation, non-hemolytic. The RIL tests showed that the O139strains possessed the same capability to cause liquide accumulation as epidemigenic strains of Vibrio cholerae O1.All O139 strains were resistant to O/129. With PCR for amplification of ctx,10 of O139 strains tested produced thesame size fragments of ctx as those of V. cholerae E1 Tor epidemigenic strains. All 23 strains isolated ftom China,India and Bangladesh possessed ctx,zot,ace and RS1 genes which were found with the molecular hybridization. Theresults showed that O139 strains were strongly virulent compared with Vibrio cholerae O1 epidemlgenic strains. Sothese strains should be considered as cholera pathogen.

关 键 词：霍乱弧菌 O139 致病性 毒力 分子生物学 

分 类 号：R378.3[医药卫生—病原生物学]