系列烷基甲基萘磺酸盐的高效液相色谱和电喷雾离子化质谱分析  

HPLC and ESI-MS Characterization of Alkylmethynaphthalene Sulfonates

作　　者：赵忠奎[1] 乔卫红[1] 付新梅[1] 李宗石[1] 程侣柏[1] 

机构地区：[1]大连理工大学精细化工国家重点实验室,辽宁大连116012

出　　处：《分析测试学报》2005年第3期10-13,共4页Journal of Instrumental Analysis

基　　金：国家973重点基础研究资助项目(G1999022501)

摘　　要：采用反相离子对高效液相色谱(HPLC)和电喷雾离子化质谱(ESI-MS)对系列长链烷基甲基萘磺酸盐(AMNS),包括己基甲基萘磺酸盐、辛基甲基萘磺酸盐、癸基甲基萘磺酸盐、十二烷基甲基萘磺酸盐、十四烷基甲基萘磺酸盐进行分析,通过液相色谱分离条件的优化,找到了最佳分析条件。紫外检测波长230nm,流动相为甲醇和水混合物,流速为0.8mL/min,采用梯度洗脱方式进行分析。线性梯度:0～40min时甲醇-水体积为60∶40,在40～70min时,甲醇-水体积比为100∶0。样品分离度高,分离效果较好。用面积归一化法得到了样品各组分的相对百分含量。HPLC和ESI-MS是快捷、有效和可靠的分析和表征AMNS的方法。Aseries of long chain alkyl methylnaphthalene sulfonates(AMNS),including hexyl methylnaphthaˉlene sulfonate,octyl methylnaphthalene sulfonate,decyl methylnaphthalene sulfonate,dodecyl methylnaphthaˉlene sulfonate,and tetradecyl methylnaphthalene sulfonate,was characterized byhigh performance liquid chroˉmatography(HPLC)and electrospray ionization mass spectrometry(ESI-MS).Through a great deal of testˉing,the analytical conditions were optimized.Detection was carried out at the wavelength of230nm.Differˉent composition mixtures of methanol and water were used as eluents with a mobile phase flow rate of0.8mL/min.The linear gradient elution was as follows:the volume ratio of methanol to water was60∶40from0to40min and100∶0from40to70min.By using the optimal analytical conditions,the target composiˉtions were separated satisfactorily.The relative amounts of the constituents were determined by an area normalˉization method.HPLC and ESI-MS are rapid,effective and reliable for the analysis of AMNS.

关 键 词：高效液相色谱 电喷雾离子化质谱 烷基甲基萘磺酸盐 表征 

分 类 号：O657.72[理学—分析化学] O657.63[理学—化学]