急性毒鼠强中毒患者体内毒物浓度及尿微量蛋白系列检测的意义  

The Detection and Clinical Significance of Poison Concentration and Urinary Microprotein in ATI Patients

作　　者：应斌宇[1] 孙来芳[1] 温怀凯[1] 倪莉 徐雪松[2] 林宏亮[1] 樊新[1] 

机构地区：[1]温州医学院附属第二医院,温州325027 [2]温州市公安局刑事科学研究所

出　　处：《临床急诊杂志》2005年第3期3-5,共3页Journal of Clinical Emergency

基　　金：本课题受温州市科委资助;课题号:S2001A23

摘　　要：目的通过测定尿液微量白蛋白(mALB)、α_1-微球蛋白(α_1-MG)、免疫球蛋白(IgG)、N-乙酰-β-D-氨基葡萄糖苷酶(NAG)含量,以观察判断毒鼠强对急性中毒患者的肾功能损害及程度。方法采用免疫速率散射比浊法及人工分光光度计比色法,对38例急性毒鼠强中毒(ATI)患者微量蛋白系列的联合检测,并与15例健康者对照。结果 ATI 患者尿 mALB、α_1-MG、IgG、NAG 排泄水平较正常对照组均有明显增加(P<0.05或0.01),且相互之间呈正相关;血清毒鼠强浓度高组(174.20±99.46μg/L)与低组(52.94±20.69μg/L)尿 mALB、α_1-MG、IgG、NAG 排泄水平有显著性差异(P<0.05);且血清毒鼠强浓度与尿 mALB、α_1-MG、NAG 排泄水平呈相关。相关结论 ATI 患者急性期存在不同程度早期肾功能损害,且与血清毒鼠强毒物浓度(TET)有关;尿微量蛋白系列检测可以早期、敏感地发现肾功能损害及程度,对重度中毒者应及时防治,有效地控制抽搐。目的通过测定尿液微量白蛋白(mALB)、α_1-微球蛋白(α_1-MG)、免疫球蛋白(IgG)、N-乙酰-β-D-氨基葡萄糖苷酶(NAG)含量,以观察判断毒鼠强对急性中毒患者的肾功能损害及程度。方法采用免疫速率散射比浊法及人工分光光度计比色法,对38例急性毒鼠强中毒(ATI)患者微量蛋白系列的联合检测,并与15例健康者对照。结果 ATI 患者尿 mALB、α_1-MG、IgG、NAG 排泄水平较正常对照组均有明显增加(P<0.05或0.01),且相互之间呈正相关;血清毒鼠强浓度高组(174.20±99.46μg/L)与低组(52.94±20.69μg/L)尿 mALB、α_1-MG、IgG、NAG 排泄水平有显著性差异(P<0.05);且血清毒鼠强浓度与尿 mALB、α_1-MG、NAG 排泄水平呈相关。相关结论 ATI 患者急性期存在不同程度早期肾功能损害,且与血清毒鼠强毒物浓度(TET)有关;尿微量蛋白系列检测可以早期、敏感地发现肾功能损害及程度,对重度中毒者应及时防治,有效地控制抽搐。Objective To observe and delide the renal function impairment and it extention in acute tetramine intoxication patients. (ATI)by detecting urinary microalbumin(mALB),α_1-microglobulin(α_1-MG),immunoglobulin G(IgG),N-acetyl-β-D-glucosmindase (NAG).Methods The urinary microprotein series in 38 ATI patients were detected by immunoturbidimetry artifieal and colorimetry.The result were compared with 15 healthy people.Results The discharging levels of urinary mALB,α_1-MG,IgG,NAG were obviously higher than those in the controls(P<0.05 or 0.01),and they were positively corredated.There were significant difference (P<0.05 or 0.001) of discharging levels between the group which had higher serum tetramine concentration and the group which had lower one in urinary mALB. α_1-MG,NAG;and they were positively corredated with patients's serum tetramine concentrations.Conclusion There are different degree of early renal function impairment in ATI patients.They are related to the serum tetramine concentrations.The detection of urinary microprotein series can help to find the renal impairment and its extention sensitively in early stage,Critical ATI patients need prevention and treatment in early time.

关 键 词：急性毒鼠强中毒 毒物浓度 肾功能 尿微量蛋白 

分 类 号：R995[医药卫生—毒理学]