果蝇对冷、热极限温度耐受能力的研究  

Effects of Cold- and Heat-hardening on Resistence to Temperature Extremes in Drosophila melanogaster

作　　者：陈小芬[1] 杨玉荣[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第B06期186-190,共5页Journal of Xiamen University：Natural Science

基　　金：教育部留学回国人员启动基金资助

摘　　要：以野生型黑腹果蝇(Drosophilamelanogaster)为基本实验材料,统计分析了非致死的冷、热预刺激对果蝇耐极限温度能力的影响,并用SDSPAGE电泳研究了冷、热刺激后热休克蛋白的表达情况.实验发现,冷、热预刺激能提高果蝇在同类温度下的耐受水平.同时,高、低温具有交互作用,即冷预刺激提高了果蝇在高温下的存活率.在0℃下,果蝇体内22、23、26、27、42、51、52及70kD的蛋白质出现增量表达;37℃下70、83kD的蛋白质表达量增多,推测上述增效作用可能是这些蛋白质引起的.The effects of cold- and heat-hardening on resistance to both low and high temperature stress was examined in Drosophila melanogaster wild-type lines.The expression of heat shock proteins was detected using SDS-PAGE electrophoresis.The hardening effect was positive when the hardening was of the same type as the stress. A cross-protection effect was also present,which is increased heat resistance after cold hardening. When flies were exposed to 0℃, proteins of 22,23,26,27,42,51,52 and 70 kD increase expression,while heat shock at 37℃ induces the added expression of 70 kD and 83 kD proteins. The results suggest that protection-effect be at least partly due to the presence of above proteins.

关 键 词：极限温度 耐受能力 SDS-PAGE电泳 热休克蛋白 蛋白质 黑腹果蝇 统计分析 实验材料 实验发现 交互作用 增效作用 野生型 热刺激 存活率 表达量 

分 类 号：S852.4[农业科学—基础兽医学] TQ351.45[农业科学—兽医学]