海洋微藻多糖的红外光谱分析初探  

Analysis of Marine Microalgal Polysaccharides by Gas Chromatography and IR Spectroscopy

作　　者：张赛金[1] 李文权[2] 邓永智[1] 陈清花[1] 陈祖峰[1] 

机构地区：[1]厦门大学海洋学系 [2]厦门大学亚热带海洋研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第B06期212-214,共3页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(39870565);福建省科技重点项目(2002Y052)资助

摘　　要：利用傅立叶变换红外光谱分析了以水为介质提取的海水小球藻(MarineChlorella)、亚心形扁藻(Platymonassubcordiformis)和球等鞭金藻(Isochrysisgalbana)多糖的主要单糖组成、环状结构、半缩醛羟基构型及取代基类型.结果表明,3种海洋微藻多糖中均含有酰氨基,葡萄糖是3种多糖的主要组成单糖.此外,海水小球藻多糖还含有硫酸基.3种海洋微藻多糖组成单糖的环构型和成苷的半缩醛羟基构型也存在较大差别.海水小球藻多糖组成单糖主要为吡喃糖,α半缩醛羟基和β半缩醛羟基两种糖苷构型共存;亚心形扁藻多糖组成单糖为吡喃糖,成苷的半缩醛羟基主要为α构型;球等鞭金藻多糖则同时含有α吡喃环和呋喃环两种构型.The chemical compositions and configurations of marine microalgal polysaccharides water-extracted from Marine Chlorella,Platymonas subcordiformis and Isochrysis galbana were studied by FT-IR spectroscopy.The results show that all the three marine microalgal polysaccharides have acylamino-radical.Glucose is always a dominant monosaccharide in the three polysaccharides.Besides,there are sulfate radical in the polysaccharide of Marine Chlorella.Pyranose is the major component of the polysaccharide in Marine Chlorella,with two glycoside configurations,α-hemiacetal hydroxy and β-hemiacetal hydroxy,coexisted.Pyranose is also the major component of the polysaccharide in Platymonas subcordiformis,with the major glycoside configuration of α-hemiacetal hydroxy.Two configurations,α-Pyranose and furanose,coexist in the polysaccharide in Isochrysis galbana.

关 键 词：红外光谱分析 海洋微藻 多糖 海水小球藻 亚心形扁藻 球等鞭金藻 傅立叶变换 单糖组成 环状结构 吡喃糖 构型 羟基 缩醛 取代基 葡萄糖 酰氨基 呋喃环 介质 糖苷 环和 

分 类 号：O657.33[理学—分析化学] Q949.2[理学—化学]