0#柴油水溶性成分对僧帽牡蛎(Ostrea cucullata)谷胱甘肽硫转移酶活性的影响  

Effect of Water-soluble Fraction of No.0 Oil on Glutathione S-transferase in Ostrea cucullata

作　　者：陈荣[1] 郑微云[1] 郁昂[1] 

机构地区：[1]近海海洋环境科学国家重点实验室(厦门大学),厦门大学环境科学研究中心,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第B06期219-221,共3页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金青年基金(20207006);厦门大学校级自选课题基金资助

摘　　要：在实验条件下,将僧帽牡蛎(Ostreacucullata)分别置于含有低(0.5mg/L)、中(2mg/L)和高(5mg/L)3种浓度0#柴油水溶性成分(watersolublefraction,WSF)的海水中,在污染后1,4,7,11,15d采样,15d后转入清洁海水中进行6d的恢复实验,采样.测定消化腺和鳃谷胱甘肽硫转移酶(GST)的活性.结果表明:正常生理条件下鳃GST活性高于消化腺;消化腺和鳃GST活性随污染时间的延长先被诱导后逐渐下降,并存在一定的剂量效应关系,可作为监测海洋石油污染的生物标志物;解除污染后,GST活性恢复到对照组水平.In experimented condition,oyster Ostrea cucullata was exposed to different concentrations of water-soluble fraction (WSF) of NO.0 diesel oil (0.5,2,5 mg/L) for 15 d,and then transferred into clean sea water for 6d restoring experiment.The glutathione S-transferase (GST) activities in the digestive gland and the gill of 1,4,7,11,15,21 d sample were measured.The results show:In control group,the gill GST is higher than digestive gland;With the prolonged exposure,the digestive gland and gill GST are induced first,and then reduce gradually.The dose-effect relationship occurres in 15 d sample;GST activity of all concentration groups are recovered to the level corresponding to that of the control group after recovery experiment.According to the data,the GST of marine bivalve could be a biomarker to monitor marine oil pollution.

关 键 词：谷胱甘肽硫转移酶 水溶性成分 僧帽牡蛎 柴油 剂量-效应关系 海洋石油污染 生物标志物 消化腺 GST 实验条件 生理条件 活性恢复 除污染 海水 采样 监测 

分 类 号：X503.225[环境科学与工程—环境工程] TS253.1[轻工技术与工程—农产品加工及贮藏工程]