双染色Th细胞测定法的初步临床应用  

Clinical application of double staining mensuration for helper T lymphocytes

作　　者：沈燕[1] 苟建军[1] 苏天水[1] 杨林芳[1] 张愔[1] 王清霞[1] 

机构地区：[1]郑州大学第一附属医院检验科,河南郑州450052

出　　处：《河南医学研究》2005年第2期138-142,共5页Henan Medical Research

基　　金：河南省科技攻关计划项目(编号:991160210)

摘　　要：目的:研究双染色Th细胞测定法(双色法)在临床Th1/Th2状态分析中的可行性。方法:以双色法、RTPCR和ELISA法分析乙型肝炎、哮喘和类风湿关节炎(RA)患者的Th1/Th2状态。结果:①乙型肝炎患者肝功能异常组的IFNγmRNA显著升高(P<0.05),肝功能正常组的IL4mRNA升高(P<0.05)。血浆IFNγ和IL4水平分析,肝功能异常组IFNγ升高(P<0.05),肝功能正常组IL4升高(P<0.05)。②RA患者血浆IL4和IL6水平显著升(P<0.05和P<0.01)。RA患者CD3+和CD4+细胞水平与对照组相比,差异无显著意义(P>0.05);CD8+/CD28-细胞水平明显低于对照组(P<0.01);CD25+细胞水平明显高于对照组(P<0.01)。RA患者CD19+细胞水平高于对照组(P<0.05)。③与正常对照组比较,不管是蛋白水平还是转录水平,哮喘患者IL4升高,IFNγ无明显变化。④双色法分析临床Th1和Th2的变化,类风湿性关节炎和哮喘患者以Th2细胞升高为主,乙型肝炎肝功能正常的患者Th2细胞升高,肝功异常组Th1细胞增加。结论:双色法可用于临床Th1/Th2分析。Objective: To investigate the feasibility of double staining mensuration for helper T lymphocytes in clinical application.Methods: Th1/Th2 lymphocytes of patients with hepatitis B, asthma or rheumatoid arthritis (RA) were determined by double staining mensuration, RT-PCR and ELISA.Results: ① At transcriptional level the expression of IFN-γmRNA was up-regulated in patients with hepatitis B whose liver function(YC group) was abnormal(P<0.05),while in patients with hepatitis B whose liver function was normal(ZC group) the expression of IL-4 mRNA was up-regulated(P<0.05).At protein level the level of IFN-γ in plasma of YC group patients was increased(P<0.05),whereas IL-4 level was elevated (P<0.05) in plasma of 2c group patients.② The levels of IL-4 and IL-6 in plasma of patients with rheumatoid arthritis were increased greatly(P<0.05 and P<0.01). There was no significant difference between patients with RA and normal control in the levels of CD3+ and CD4+ lymphocytes (P>0.05). And CD8+/CD28- lymphocytes were significantly reduced(P<0.01).CD25+ and CD19+ lymphocytes were both elevated (P<0.01 and P<0.05).③ The level of IL-4 in patients with asthma was higher than that of control, whereas there was no significant difference between patients with asthma and normal control in the level of IFN-γ.④ By double staining mensuration for helper T lymphocytes Th2 lymphocytes were dominantly appeared in patients with rheumatoid arthritis or asthma. Th2 cells in ZC group patients with hepatitis B were increased as well as Th1 cells in YC group patients with hepatitis B.Conclusion: Double staining mensuration for helper T lymphocytes could analyse Th1/Th2 cells in clinical application.

关 键 词：双染色Th细胞测定法 TH1/TH2 乙型肝炎 哮喘 类风湿关节炎 

分 类 号：R392.3[医药卫生—免疫学]