成鼠骨髓间充质干细胞体外分化为神经前体细胞的研究  

Differentiation of mesenchymal stem cells in adult rats into neural precursor cells in vitro

作　　者：刘宏志[1] 袁维[2] 路磊[1] 

机构地区：[1]中国医科大学附属第一医院骨科 [2]上海市第八人民医院骨科

出　　处：《中国医科大学学报》2005年第3期212-213,共2页Journal of China Medical University

基　　金：辽宁省博士启动基金资助项目(20021031)

摘　　要：目的:探讨成年大鼠骨髓间充质干细胞(MSC)体外分化为神经前体细胞的可能性。方法:MSC原代培养、传代后在细胞因子和氧化剂作用下进行诱导。形态学观察,免疫细胞化学、流式细胞分析和细胞电生理检查。结果:MSC体外可诱导为神经前体细胞,诱导后有表面抗原nestin表达,5h分化率49.8%,同时具有早期神经干细胞电流特性。结论:骨髓来源的神经前体细胞治疗中枢神经系统疾病成为可能。Objective: To explore the possibility of transdifferentiation of bone marrow mesenchymal stem cells (MSC) of adult rat to neural precursor cells in vitro. Methods: After the primary culture and subculture differentiation of MSC was induced into neural precursor cells by cytokine and oxidane . Gross observation, immunocytochemistry analysis, flow cytometry analysis, and electrophysiological examination were performed. Results: The MSC could be transdifferentiated to neural precursor cell with the expression of Nestin. The inducible rate of MSC was 49.8%. The induced cells showed different electrophysiological features from the MSC,but similar to the neural stem cells. Conclusion: Neural precursor cells derived from bone marrow have the possibility in the therapy of central nervous system damage.

关 键 词：骨髓 间充质干细胞 诱导分化 神经前体细胞 

分 类 号：R681[医药卫生—骨科学] R338[医药卫生—外科学]