人白细胞介素6基因转移的成纤维细胞对自然杀伤活性的增强作用  

AUGMENTATION OF NATURAL KILLER ACTIVITY BYFIBROBLASTS TRANSFECTED WITH RECOMBINANTHUMAN IL-6 GENE

作　　者：章卫平[1] 曹雪涛[1] 顾申[1] 周正芳[1] 郑玲莉[1] 叶天星[1] 

机构地区：[1]上海第二军医大学免疫学教研室

出　　处：《中华血液学杂志》1994年第6期300-303,共4页Chinese Journal of Hematology

基　　金：上海市科技启明星基金

摘　　要：应用磷酸钙共沉淀法将人白细胞介素６（ＩＬ－６）基因转入成纤维细胞ＨＩＨ３Ｔ３，经Ｇ４１８抗性筛选和有限稀释法，从１２株阳性细胞克隆中选出一株高分泌ＩＬ－６克隆（代号为Ｔ６．６）。Ｔ６．６培养上清能显著增强自然杀伤（ＮＫ）活性，当与ＩＬ－２合用时增强效果更佳，抗ＩＬ－６单抗能阻断这种增强作用。将Ｔ６．６大量扩增后植入小鼠腹腔，４８小时后血清中可检测出较高水平的ＩＬ－６，且脾细胞ＮＫ活性比对照组显著升高。实验结果表明，人ＩＬ－６基因转移的成纤维细胞在体内、外均能显著增强ＮＫ活性，提示成纤维细胞介导的ＩＬ－６基因疗法可应用于抗肿瘤、抗感染和造血功能重建等。ecombinant human IL-6(rhIL-6)gene was trans-fected into NIH3T3 fibroblasts by calcium phosphate co-precipitation method.A fibroblast cell line ( T6.6)which secreted IL-6 at highest level was selected from 12positive cell clones by G418 resistance selection and limi-ting dilution. The culture supernatant(SN)of T6.6could augment NK activity significantly and the augmen-tation was more efficient on combination with IL-2.Theaugmentation could be blocked by anti-IL- 6 MoAb.When T6. 6 was implanted i.p. into mice, high levels ofIL-6 could be detected in sera in 48 hours and splenicNK activity was enhanced significantly. These resultsdemonstrated that fibroblasts transfected with rhIL- 6gene could significantly augment NK activity in vitro andin vivo,and indicated fibroblasts mediated IL- 6 genetherapy could be applied to the cancer and infection treatment.

关 键 词：白细胞介素6 成纤维细胞 自然杀伤 

分 类 号：R456[医药卫生—治疗学]