人脐血造血干、祖细胞体外培养的实验研究  

IN VITRO CULTURE OF HEMOPOIETIC STEM IPRO-GENITOR CELLS IN HuMAN UMBILICAL CORD BLOOD

作　　者：马立群[1] 谭汉君[1] 冉崇蓉[1] 

机构地区：[1]北京解放军总医院

出　　处：《中华血液学杂志》1994年第11期583-585,共3页Chinese Journal of Hematology

摘　　要：采用体外细胞培养的方法，检测了脐血、骨髓、外周血ＣＦＵ－ＧＭ，ＢＦＵ－Ｅ。结果表明脐血中ＣＦＵ－ＧＭ，ＢＦＵ－Ｅ数量高于成人外周血，同骨髓相比，ＣＦＵ－ＧＭ数量无显著差异，ＢＦＵ－Ｅ低于骨髓。同时观察到脐血中ＣＦＵ－ＧＭ大集落比例明显高于骨髓；ＣＦＵ－ＧＭ集落产率时间曲线较骨髓延迟３～４天；Ｓ期ＧＭ－ＣＦＣ所占比例明显低于骨髓，可能提示脐血造血细胞中早期造血细胞比例高于骨髓。实验中还发现脐血红系集落生长同骨髓十分相似，二者均含ＣＦＵ－Ｅ和ＢＦＵＥ集落，而外周血中只含ＢＦＵ－Ｅ集落。我们统计了２７份脐血血量及所含有核细胞数。根据骨髓移植的有核细胞数标准（２×１０￣８／ｋｇ），一份脐血的有核细胞数很难达到此要求，但已达到目前国外小儿异体脐血移植成功病例的有核细胞数值。he capability of human umbilical cord blood (UCB)as a sourcee of transplantable stemlprogenitor cells wasevaluated in comparison with bone marrow(BM) and pe-ripheral blood(PB) by cell culture in vitro. The numberof CFUGM and BFUE in UCB was significantly high-er than that in PB. CFU-GM between BM and UCBhad no statistical difference, but BFU-E was lower inUCB.The percentage of large CFUGM colonies (>1000cells)was 43. 3%in UCB which was significantly higherthan that in BM (3. 6% ,P<0. 01). Maybe there ishigher percentage of imrnature hemopoietic cells in UCBthan that in BM. As compared with BM, the growthpeak of CFU-GM colony of UCB delayed about 3～4days. The percentage of GM-CFC in S phase was23. 23%in UCB, however it was much higher in BM(38- 85%, P<0.01). The growth pattern of BFU-Ecolonies showed a continuous spectrum of erythroid pro-genitor cells in UCB and BM , whereas, CFU-E couldn't be found in PB.The volume of one cord blfor was 30～120ml(mean63. 5±27. 16 ml, not including placental blood). Thenucleated cell count was 1. 83×10￣7/ml. It just met theneeds of UCBtransplantation in children. Hemopoiesisvvas successfuly recons tituted in one case who receivednucleated cells as low as<0. 4×10￣8/kg.

关 键 词：脐血 造血祖细胞 体外培养 造血干细胞 脐血移植 

分 类 号：R457.9[医药卫生—治疗学]