微量血清等电聚焦电泳及免疫印迹法测定人载脂蛋白E表型  

Phenotyping of human apolipoprotein E from micro-serum by isoelectric focusing and immunoblotting

作　　者：吕新跃[1] 陈保生[1] 薛红[1] 张东升[1] 贾振华 许秀荣 

机构地区：[1]天津武警医学专科学校,中国医学科学院基础医学研究所,河南医科大学,河南商邱市人民医院

出　　处：《中华医学检验杂志》1994年第1期26-28,共3页

摘　　要：应用制备的１株抗人血清载脂蛋白Ｅ（ａｐｏＥ）单克隆抗体作为一抗，通过微量脱脂血清等电聚焦电泳（ＩＥＦ）及免疫印迹法检测ａｐｏＥ表型。与常规方法比较，本法不需超速离心分离人血清极低密度脂蛋白（ＶＬＤＬ），仅需新鲜或－２０℃冻存数周的微量血清，故此法简便易于临床应用。采用本法，作者分析了部分正常人的ａｐｏＥ表型及其等位基因频率分布，所得结果与常规法一致。he microliters of delipidated serum was applied to an isoelectric focusing gel electrophoresis where-after the apolipoprotein E polymorphism was visualized by immunoblotting using monoclonal anti-apolipoprotein E antibody prepared as first antibody.Compared with conventional method,the present method is easily used for clinical diagnosis because ultracentrifugation to isolate VLDL is not needed.A few microliters offresh serum or samples stored at-20℃ for 4～6weeks are good enough for the tests.Authors have analysed the distribution of apo E phenotypes from 94 normal subjects.The same results were obtained with the present method and conventional method.

关 键 词：载脂蛋白E 表型 免疫印迹法 抗体 

分 类 号：R446.112[医药卫生—诊断学]