AA和MDS患者骨髓CD_(34)^+细胞及G-CSFR的表达及意义  

The expression and significance of CD_(34)^+ cells and G-CSFR on CD_(34)^+ cells in bone marrow of the patients with AA and MDS

作　　者：李爱[1] 许洪志[1] 刘新[1] 王相华[1] 

机构地区：[1]山东大学山东省立医院,山东济南250021

出　　处：《山东医药》2005年第16期6-8,共3页Shandong Medical Journal

摘　　要：目的检测再生障碍性贫血(AA)和骨髓增生异常综合征(MDS)患者骨髓CD3+4细胞占单个核细胞(MNC)的比率及其表面粒细胞集落刺激因子受体(G-CSFR)的表达率,以探讨二者可能的发病机制。方法用流式细胞术(FCM)检测13例AA、22例MDS及12例非血液病患者骨髓CD3+4细胞占MNC的比率及其表面G-CSFR的表达率。结果AA组与对照组、AA组与MDS组、MDS-难治性贫血(RA)组与MDS-难治性贫血伴原始细胞增多(RAEB)组的骨髓MNC中CD3+4细胞比率比较有显著性差异(P<0.05),但G-CSFR的表达率比较无显著性差异(P>0.05)。大多数重型AA(SAA)患者(3/4)及很少慢性AA(CAA)患者(1/9)的骨髓MNC中CD3+4细胞比率小于0.1%。大多数G-CSFR表达率低(<14%)的MDS患者(7/9)外周血中性粒细胞减少;中性粒细胞减少在G-CSFR表达率正常(14%~28.9%)的患者(1/6)很少见;G-CSFR表达率高(>28.9%)的患者(3/7)也存在中性粒细胞减少。结论骨髓CD3+4细胞检测有助于判断AA患者病情及MDS患者的预后,亦可用于鉴别AA和MDS。Objective To detect the ratio of CD^+_ 34 cells in bone marrow mononuclear cells and the expressive rate of granulocyte colony-stimulating factor receptor(G-CSFR) on CD^+_ 34 cells in bone marrow of the patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS),so to explore different pathogenesis between AA and MDS.Method The ratio of CD^+_ 34 cells in bone marrow mononuclear cells and the expressive rate of G-CSFR on CD^+_ 34 cells of 13 AA patients,22 MDS patients and 12 normal subjects were detected by flow cytometry (FCM).Results There was significant difference according to the ratio of CD^+_ 34 cells in mononuclear cells of bone marrow between AA group and control group,AA group and MDS group,MDS-RA group and MDS-RAEB group (P<0.05) but there was no significant difference according to the expressive rate of G-CSFR on CD^+_ 34 cells (P>0.05).The ratio of CD^+_ 34 cells in mononuclear cells of bone marrow was less than 0.1% in the majority of SAA patients (3/4) while it was seldom less than 0.1% in CAA patients (1/9).Most MDS patients (7/9) with low G-CSFR expression had neutropenia of the peripheral blood.Neutropenia was less common in the normal group (1/6),but also occurred in the high expression group (3/7).Conclusion The determination of CD^+_ 34 cells can be used to judge pathogenetic condition of AA patients and the prognosis of MDS patients.It also is helpful to distinguish AA and MDS.

关 键 词：骨髓CD34^+细胞 骨髓增生异常综合征(MDS) AA 粒细胞集落刺激因子受体 中性粒细胞减少 流式细胞术(FCM) 表达及 再生障碍性贫血 显著性差异 难治性贫血 原始细胞增多 表达率 单个核细胞 血液病患者 MNC 发病机制 细胞检测 

分 类 号：R259.565[医药卫生—中西医结合] R446.11[医药卫生—中医内科学]