HPLC双波长法测定一清胶囊中黄芩苷、大黄素的含量  

Determination of baicalin and emodin in Yiqing Capsule by HPLC

作　　者：张科卫[1] 李伟[1] 蔡皓[1] 崔小兵[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《中成药》2005年第6期653-656,共4页Chinese Traditional Patent Medicine

摘　　要：目的:研究以HPLC法同时测定一清胶囊(黄芩、大黄)中黄芩苷、大黄素含量的方法。方法:采用KromasilC18柱,以甲醇-0.2磷酸为流动相,梯度洗脱,双波长检测,λ1=315nm,λ2=266nm。结果:黄芩苷在64.40～322.00μg·mL-1范围内线性关系良好,r=0.9998,大黄素在0.82～13.20μg·mL-1范围内线性关系良好,r=0.9999。平均加样回收率:黄芩苷为99.96,RSD为1.57(n=9);大黄素为98.90,RSD为1.25(n=9)。结论:本法操作简便,结果可靠,重现性好。AIM: To establish a HPLC for determination of baicalin and emodin in Yiqing Capsule(Radix Scutellarial, Radix et Rhizoma Rhei). METHODS:A Kromasil C_(18) Column was used. The mobile phase was methanol -0.2% phosphoric acid for gradient elution. Baicalin and emodin were determined by dual wavelength, λ_1=315 nm, λ_2=266 nm. RESULTS:The linear range of baicalin was within 64.40-322.00 μg·mL^(-1) (r=(0.999 8)). The average recovery was 99.96%,RSD was 1.57%(n=9). The linear range of emodin was within( 0.82)-13.20 μg·mL^(-1)(r=0.999 9).The average revovery was 98.90%, RSD was 1.25%(n=9). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control in production of Yiqing Capsule. (Nan

关 键 词：HPLC 一清胶囊 双波长 黄芩苷 大黄素 

分 类 号：R927.2[医药卫生—药学]