MECC法分离中药熊胆中磷脂主组分方法的建立  

Separation of phospholipid in bear gall by Micellar electrokinetic capillary chromatography

作　　者：董颖[1] 卢学清[2] 王智华[2] 

机构地区：[1]复旦大学附属中山医院,上海200032 [2]上海中医药大学,上海201203

出　　处：《中成药》2005年第6期713-718,共6页Chinese Traditional Patent Medicine

摘　　要：目的:建立胶束电动毛细管电泳(MECC)分离分析中药熊胆中磷脂主组分的方法。方法:采用毛细管电泳(HPCE)方法,详细观察了各因素对分离的影响。所确定的操作条件为:缓冲液为35mMSDC、6mM硼砂、10mM磷酸二氢钠、30%(v/v)正丙醇,pH8.5:操作电压为30kV;温度40°C;毛细管长度570mm,有效长度500mm;紫外检测波长200nm;进样压力50mbar×4秒。结果:6个熊胆样品中的磷脂主组分在最佳MECC操作条件下获得分离。结论:本法快速、高效,并与HPLC互补。AIM: To develop micellar electrokinetic capilitary chromatography. A method MECC for the separation of major phospholipid from bear gall. METHODS: Following the optimization experiments the MECC was applied to the separation with the 35 m MSDC, 6 mM sodium tetraborate, 10 mM disodium hydrogen phosphate, 30% (v/v) 1-propanol, and pH 8.5 as buffer system. The voltage was 30 kV. the temperature was set at 40 (°C). The capillary length was 570 mm with the effective one of 500 mm. Injection pressure was 50 mbar×4 s and UV detection wavelength was at 200 nm. RESULTS: The separation of phospholipid in bear gall was achieved in the method developed. CONCLUSION: The method is fast, effective and complementary to the HPLC.

关 键 词：熊胆 磷脂类化合物 MECC 

分 类 号：R284.2[医药卫生—中药学]